Identification of Genes Induced by Growth Hormone in Rat Liver Using cDNA Arrays

Thompson, Barry J. L., Shang, Catherine A. and Waters, Mike J. (2000) Identification of Genes Induced by Growth Hormone in Rat Liver Using cDNA Arrays. Endocrinology, 141 11: 4321-4324. doi:10.1210/en.141.11.4321


Author Thompson, Barry J. L.
Shang, Catherine A.
Waters, Mike J.
Title Identification of Genes Induced by Growth Hormone in Rat Liver Using cDNA Arrays
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
Publication date 2000-11-01
Year available 2000
Sub-type Article (original research)
DOI 10.1210/en.141.11.4321
Open Access Status Not yet assessed
Volume 141
Issue 11
Start page 4321
End page 4324
Total pages 4
Editor K.S. Korach
Place of publication Bethesda, MD, USA
Publisher Endocrine Society
Language eng
Subject C1
270000 Biological Sciences
730000 - Health
Abstract Growth hormone (GH) is a pleiotropic cytokine that acts upon its target cells to regulate their growth, differentiation and metabolism. GH is thought to act by altering gene expression in target cells, but few GH-regulated genes are known. In this study, we used cDNA array analysis to identify genes rapidly induced in the liver of GH-deficient dwarf rats following a single systemic injection of GH. Eight genes were found to upregulate their mRNA expression within 1-3 hours of GH administration, results which were confirmed by northern analysis. The identity of these genes suggests GH may influence a diversity of cellular processes. A role for GH in regulating cytokine and growth factor signalling is suggested by upregulation of mRNAs encoding three signal transducers: a subunit of the receptor for IL-6-type cytokines (gp130), STAT3 (signal transducer and activator of transcription) and p38MAPK (mitogen activated protein kinase). Two genes involved in DNA repair and cell cycle control, APEN (apurinic endonuclease) and GADD45 (growth arrest and DNA damage 45) were upregulated. Other induced genes include those encoding a lactate transporter (MCT-1), an extracellular matrix remodelling enzyme, MT1-MMP (membrane type 1 matrix metalloproteinase) and an acute phase protein (fibrinogen beta). In summary, this work is the first to apply cDNA arrays to the study of peptide hormone action in vivo and has identified 8 novel GH target genes.
Keyword Endocrinology & Metabolism
Acute-phase Response
Messenger-rna
Protein
Expression
Binding
Mice
Ref-1
P450
Q-Index Code C1
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Tue, 10 Jun 2008, 22:31:41 EST