Toward larger chemical libraries: Encoding with fluorescent colloids in combinatorial chemistry

Battersby, Bronwyn J., Bryant, Darryn, Meutermans, Wim, Matthews, Daniel, Smythe, Mark L. and Trau, Matt (2000) Toward larger chemical libraries: Encoding with fluorescent colloids in combinatorial chemistry. Journal of the American Chemical Society, 122 9: 2138-2139. doi:10.1021/ja993634i


Author Battersby, Bronwyn J.
Bryant, Darryn
Meutermans, Wim
Matthews, Daniel
Smythe, Mark L.
Trau, Matt
Title Toward larger chemical libraries: Encoding with fluorescent colloids in combinatorial chemistry
Journal name Journal of the American Chemical Society   Check publisher's open access policy
ISSN 0002-7863
Publication date 2000-02-17
Sub-type Article (original research)
DOI 10.1021/ja993634i
Volume 122
Issue 9
Start page 2138
End page 2139
Total pages 2
Editor A.J. Bard
Place of publication Washington, USA
Publisher American Chemical Society
Language eng
Subject C1
250103 Colloid and Surface Chemistry
780103 Chemical sciences
Abstract Chemical library technology plays a central role in research areas such as drug discovery and gene screening.1 The most powerful combinatorial library synthesis method is the iterative “split and mix” synthesis on insoluble microscopic beads.2 This technique is an efficient method for accessing all combinations of chosen monomers such as nucleic acids, amino acids, or sugars in a small number of reactions. Compound identification from such large pools of compounds, be it bound to resin (one compound per bead) or in solution, is achieved through covalent attachment of molecular tags to the beads3 or through iterative deconvolution technologies.4 Herein we introduce an encoding method that involves physically attaching fluorescent colloidal particles (“reporters”) to the surface of solid support beads during split and mix syntheses, to produce an information-rich, colored barcode that can be easily, rapidly, and inexpensively decoded using fluorescence microscopy. This “colloidal barcoding” technique eliminates the need for compatible tagging chemistry in conventional molecular tagging3 or optical encoding5 procedures and permits unambiguous identification of compounds in libraries of any size and type.
Keyword Chemistry, Multidisciplinary
Synthetic Peptide Library
Drug Discovery
Generation
Tags
combinatorial library synthesis method
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Physical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 121 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 127 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 10 Jun 2008, 22:25:20 EST