Single DNA sequence common to all chlamydial species employed for PCR detection of these organisms

Girjes, A., Carrick, F. N. and Lavin, M. F. (1999) Single DNA sequence common to all chlamydial species employed for PCR detection of these organisms. Research in Microbiology, 150 7: 483-489. doi:10.1016/S0923-2508(99)00108-4


Author Girjes, A.
Carrick, F. N.
Lavin, M. F.
Title Single DNA sequence common to all chlamydial species employed for PCR detection of these organisms
Journal name Research in Microbiology   Check publisher's open access policy
ISSN 0923-2508
Publication date 1999-01-01
Sub-type Article (original research)
DOI 10.1016/S0923-2508(99)00108-4
Volume 150
Issue 7
Start page 483
End page 489
Total pages 7
Place of publication Paris, France
Publisher Elsevier
Language eng
Subject C1
321003 Cardiology (incl. Cardiovascular Diseases)
730106 Cardiovascular system and diseases
270304 Infectious Agents
730101 Infectious diseases
Abstract Chlamydial infection is responsible for a wide spectrum of diseases of the eye, genitourinary tract, and lung. This group of organisms is also implicated in the pathogenesis of coronary artery disease as well as arthritis. Since cross-species infection is widely reported (though probably underestimated), it is an advantage to have a rapid and reliable method to detect all forms of chlamydiae in patient samples. We have identified a 160/163-bp DNA fragment in Chlamydia which is highly conserved in all chlamydial species. A polymerase chain reaction method based on this sequence has been developed to detect, in clinical samples, chlamydiae which have been shown to be positive by fluorescent-staining immunoassay; this method can be utilized in combination with restriction endonuclease cleavage to identify individual chlamydial species. Thus we have developed a sensitive and rapid detection method and have used it on samples from patients with respiratory and genital infections. (C) 1999 Editions scientifiques et medicales Elsevier SAS.
Keyword Microbiology
Chlamydia
Pcr
Pathogen Detection
Universal Primers
Polymerase Chain-reaction
Koala Type-i
Trachomatis
Diagnosis
Infection
Psittaci
Specimens
Pneumonia
Disease
Cells
Q-Index Code C1

 
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Created: Tue, 10 Jun 2008, 22:02:08 EST