An efficient extraction method from blood clots for studies requiring both host and viral DNA

Basuni, A. A., Butterworth, L. A., Cooksley, G., Locarnini, S. and Carman, W. F. (2000) An efficient extraction method from blood clots for studies requiring both host and viral DNA. Journal of Viral Hepatitis, 7 3: 241-243.

Author Basuni, A. A.
Butterworth, L. A.
Cooksley, G.
Locarnini, S.
Carman, W. F.
Title An efficient extraction method from blood clots for studies requiring both host and viral DNA
Journal name Journal of Viral Hepatitis   Check publisher's open access policy
ISSN 1352-0504
Publication date 2000-01-01
Sub-type Article (original research)
Volume 7
Issue 3
Start page 241
End page 243
Total pages 3
Place of publication Oxford, UK
Publisher Blackwell Science
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract The clot from blood is usually discarded after the collection of serum. Yet, it contains nucleated white blood cells and substantial serum. Here, we have compared four methods to enable quick and efficient extraction of human genomic and viral DNA from clotted blood. Two of these methods, a phenol-based in-house method and Tripure isolation reagent, only achieved a low polymerase chain reaction (PCR) yield. In contrast, the QIAamp blood kit and the High Pure Viral Nucleic Acid kit were equally efficient, with similar sensitivity to serum for extraction of viral DNA.
Keyword Gastroenterology & Hepatology
Infectious Diseases
Virology
Blood Clots
Dna Extraction
Hepatitis B Virus
Human Beta-globin
Hepatitis-b
Whole-blood
Direct Pcr
Association
Clearance
Virus
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 10 Jun 2008, 21:26:41 EST