Enhanced expression of osteocalcin mRNA in human osteoarthritic trabecular bone of the proximal femur is associated with decreased expression of interleukin-6 and interleukin-11 mRNA

Kuliwaba, Julia S., Findlay, David M., Atkins, Gerald J., Forwood, Mark R. and Fazzalari, Nicola L. (2000) Enhanced expression of osteocalcin mRNA in human osteoarthritic trabecular bone of the proximal femur is associated with decreased expression of interleukin-6 and interleukin-11 mRNA. Journal of Bone and Mineral Research, 15 2: 332-341. doi:10.1359/jbmr.2000.15.2.332


Author Kuliwaba, Julia S.
Findlay, David M.
Atkins, Gerald J.
Forwood, Mark R.
Fazzalari, Nicola L.
Title Enhanced expression of osteocalcin mRNA in human osteoarthritic trabecular bone of the proximal femur is associated with decreased expression of interleukin-6 and interleukin-11 mRNA
Journal name Journal of Bone and Mineral Research   Check publisher's open access policy
ISSN 0884-0431
1523-4681
Publication date 2000-02-01
Sub-type Article (original research)
DOI 10.1359/jbmr.2000.15.2.332
Volume 15
Issue 2
Start page 332
End page 341
Total pages 10
Place of publication Washington, DC, U.S.A.
Publisher American Society of Bone and Mineral Research
Collection year 2000
Language eng
Subject C1
321017 Orthopaedics
730114 Skeletal system and disorders (incl. arthritis)
Formatted abstract
Few studies have investigated the factors or mechanisms that may lead to structural changes in OA bone. This study examines the in vivo expression of messenger RNA encoding the osteoclastogenic cytokines interleukin-6 (IL-6) and interleukin-ll (IL-11), together with the osteoblastic marker osteocalcin (OCN) and the calcitonin receptor (CTR), which in bone is exclusively expressed by osteoclasts. Total RNA was isolated from intertrochanteric trabecular bone from OA patients, and from controls taken at autopsy. The patterns of mRNA expression of IL-6, IL-11, OCN, and CTR were examined using reverse-transcription polymerase chain reaction (RT-PCR) by determining the relative ratios of the amplified products with respect to glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Both IL-6 and IL-11 mRNA were significantly less abundant in OA than in the control group. Expression of IL-11 mRNA decreased significantly with age for both groups. OCN mRNA expression was significantly more abundant in OA, and there was no significant difference for CTR mRNA between the two groups. For both OCN and CTR in OA, expression increased significantly with increasing age. These differences in expression between the OA and control groups are consistent with an hypothesis that biochemical and genetic factors in bone can contribute or perhaps underlie the degenerative joint changes seen in OA.
© 2000 American Society for Bone and Mineral Research
Keyword Endocrinology & Metabolism
Osteoarthritis
Cytokine Expression
Osteocalcin
Proximal Femur
Trabecular Bone
Osteoclast Development
Iliac Crest
Radiographic Osteoarthritis
Generalized Osteoarthritis
Subchondral Bone
Pagets-disease
Growth-factors
Stromal Cells
Femoral-neck
In-vitro
Q-Index Code C1
Additional Notes Presented in part at the 8th Annual Scientific Meeting of the Australian and New Zealand Bone and Mineral Society, Perth, Western Australia, August 1998, and the Second Joint Meeting of the American Society for Bone and Mineral Research-International Bone and Mineral Society, San Francisco, California, December 1998.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Tue, 10 Jun 2008, 21:15:20 EST