Preparation and characterization of polyclonal antibodies against human chaperonin 10

Somodevilla-Torres, Maria J., Hillyard, Narelle C., Morton, Halle, Alewood, Dianne, Halliday, Judy A., Alewood, Paul F., Vesey, David A., Walsh, Michael D. and Cavanagh, Alice C. (2000) Preparation and characterization of polyclonal antibodies against human chaperonin 10. Cell Stress and Chaperones, 5 1: 14-20. doi:10.1379/1466-1268(2000)005<0014:PACOPA>2.0.CO;2


Author Somodevilla-Torres, Maria J.
Hillyard, Narelle C.
Morton, Halle
Alewood, Dianne
Halliday, Judy A.
Alewood, Paul F.
Vesey, David A.
Walsh, Michael D.
Cavanagh, Alice C.
Title Preparation and characterization of polyclonal antibodies against human chaperonin 10
Journal name Cell Stress and Chaperones   Check publisher's open access policy
ISSN 1355-8145
1466-1268
Publication date 2000-01-01
Sub-type Article (original research)
DOI 10.1379/1466-1268(2000)005<0014:PACOPA>2.0.CO;2
Volume 5
Issue 1
Start page 14
End page 20
Total pages 7
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Language eng
Subject C1
320203 Humoral Immunology and Immunochemistry
780105 Biological sciences
Formatted abstract
Early pregnancy factor (EPF) has been identified as an extracellular homologue of chaperonin 10 (Cpn10), a heat shock protein that functions within the cell as a molecular chaperone. Here, we report the production of polyclonal antibodies directed against several different regions of the human Cpn10 molecule and their application to specific protein quantitation and localization techniques. These antibodies will be valuable tools in further studies to elucidate the mechanisms underlying the differential spatial and temporal localization of EPF and Cpn10 and in studies to elucidate structure and function.
Keyword Cell Biology
Early-pregnancy factor
Monoclonal-antibodies
Mice
Identification
Immunization
Invivo
Growth
EPF
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Tue, 10 Jun 2008, 20:33:24 EST