Improved Success of Sparse Matrix Protein Crystallization Screening with Heterogeneous Nucleating Agents

Thakur, A., Robin, G., Guncar, G., Saunders, N., Newman, J., Martin, J. L. and Kobe, B. (2007) Improved Success of Sparse Matrix Protein Crystallization Screening with Heterogeneous Nucleating Agents. PLoS ONE, 2 10: e1091-1-e1091-6. doi:10.1371/journal.pone.0001091


Author Thakur, A.
Robin, G.
Guncar, G.
Saunders, N.
Newman, J.
Martin, J. L.
Kobe, B.
Title Improved Success of Sparse Matrix Protein Crystallization Screening with Heterogeneous Nucleating Agents
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2007-10-31
Year available 2007
Sub-type Article (original research)
DOI 10.1371/journal.pone.0001091
Open Access Status DOI
Volume 2
Issue 10
Start page e1091-1
End page e1091-6
Total pages 6
Place of publication United States of America
Publisher Public Library of Science
Language eng
Subject 250302 Biological and Medical Chemistry
C1
780105 Biological sciences
Abstract Crystallization is a major bottleneck in the process of macromolecular structure determination by X-ray crystallography. Successful crystallization requires the formation of nuclei and their subsequent growth to crystals of suitable size. Crystal growth generally occurs spontaneously in a supersaturated solution as a result of homogenous nucleation. However, in a typical sparse matrix screening experiment, precipitant and protein concentration are not sampled extensively, and supersaturation conditions suitable for nucleation are often missed.
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Thu, 08 May 2008, 23:46:40 EST by Darryl Greensill on behalf of School of Chemistry & Molecular Biosciences