Robos are required for the correct targeting of retinal ganglion cell axons in the visual pathway of the brain

Plachez, Céline, Andrews, William, Liapi, Anastasia, Knoell, Bernd, Drescher, Uwe, Mankoo, Baljinder, Zhe, Liu, Mambetisaeva, Elvira, Annan, Adelaide, Bannister, Lawrence, Parnavelas, John G., Richards, Linda J. and Sundaresan, Vasi (2008) Robos are required for the correct targeting of retinal ganglion cell axons in the visual pathway of the brain. Molecular and Cellular Neuroscience, 37 4: 719-730. doi:10.1016/j.mcn.2007.12.017

Author Plachez, Céline
Andrews, William
Liapi, Anastasia
Knoell, Bernd
Drescher, Uwe
Mankoo, Baljinder
Zhe, Liu
Mambetisaeva, Elvira
Annan, Adelaide
Bannister, Lawrence
Parnavelas, John G.
Richards, Linda J.
Sundaresan, Vasi
Title Robos are required for the correct targeting of retinal ganglion cell axons in the visual pathway of the brain
Journal name Molecular and Cellular Neuroscience   Check publisher's open access policy
ISSN 1044-7431
Publication date 2008-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.mcn.2007.12.017
Open Access Status
Volume 37
Issue 4
Start page 719
End page 730
Total pages 11
Editor F. S. Walsh
Place of publication United States
Publisher Academic Press
Language eng
Subject C1
270210 Neurogenetics
730104 Nervous system and disorders
Abstract Axonal projections from the retina to the brain are regulated by molecules including the Slit family of ligands [Thompson, H., Barker, D., Camand, O., Erskine, L., 2006a. Slits contribute to the guidance of retinal ganglion cell axons in the mammalian optic tract. Dev. Biol. 296, 476–484, Thompson, H., Camand, O., Barker, D., Erskine, L., 2006b. Slit proteins regulate distinct aspects of retinal ganglion cell axon guidance within dorsal and ventral retina. J. Neurosci. 26, 8082–8091]. However, the roles of Slit receptors in mammals, (termed Robos), have not been investigated in visual system development. Here we examined Robo1 and 2 mutant mice and found that Robos regulate the correct targeting of retinal ganglion cell (RGC) axons along the entire visual projection. We noted aberrant projections of RGC axons into the cerebral cortex, an area not normally targeted by RGC axons. The optic chiasm was expanded along the rostro-caudal axis (similar to Slit mutant mice, Plump, A.S., Erskine, L., Sabatier, C., Brose, K., Epstein, C.J., Goodman, C.S., Mason, C.A., Tessier-Lavigne, M., 2002. Slit1 and Slit2 cooperate to prevent premature midline crossing of retinal axons in the mouse visual system. Neuron 33, 219–232), with ectopic crossing points, and some axons projecting caudally toward the corticospinal tract. Further, we found that axons exuberantly projected into the diencephalon. These defects were more pronounced in Robo2 than Robo1 knockout animals, implicating Robo2 as the predominant Robo receptor in visual system development.
Keyword Slit
Optic chiasm
Axon guidance
Retinal ganglion cells
Q-Index Code C1
Q-Index Status Confirmed Code
Grant ID 074549
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
Queensland Brain Institute Publications
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 26 times in Scopus Article | Citations
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Created: Wed, 07 May 2008, 18:55:58 EST by Timothy Hazelton on behalf of Queensland Brain Institute