Unlocking hidden genomic sequence

Keith, Jonathan M., Cochran, Duncan A. E., Lala, Gita H., Adams, Peter, Bryant, Darryn and Mitchelson, Keith R. (2004) Unlocking hidden genomic sequence. Nucleic Acids Research, 32 3: e35. doi:10.1093/nar/gnh022


Author Keith, Jonathan M.
Cochran, Duncan A. E.
Lala, Gita H.
Adams, Peter
Bryant, Darryn
Mitchelson, Keith R.
Title Unlocking hidden genomic sequence
Journal name Nucleic Acids Research   Check publisher's open access policy
ISSN 0305-1048
1362-4962
Publication date 2004-02-18
Sub-type Article (original research)
DOI 10.1093/nar/gnh022
Open Access Status DOI
Volume 32
Issue 3
Start page e35
Total pages 9
Editor M. Geit
Place of publication United Kingdom
Publisher Oxford University Press
Language eng
Subject 230101 Mathematical Logic, Set Theory, Lattices And Combinatorics
Abstract Despite the success of conventional Sanger sequencing, significant regions of many genomes still present major obstacles to sequencing. Here we propose a novel approach with the potential to alleviate a wide range of sequencing difficulties. The technique involves extracting target DNA sequence from variants generated by introduction of random mutations. The introduction of mutations does not destroy original sequence information, but distributes it amongst multiple variants. Some of these variants lack problematic features of the target and are more amenable to conventional sequencing. The technique has been successfully demonstrated with mutation levels up to an average 18% base substitution and has been used to read previously intractable poly(A), AT-rich and GC-rich motifs.
Keyword Random mutagenesis
Sanger sequencing
Genomic sequence
Q-Index Code C1

 
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Created: Thu, 10 May 2007, 00:05:32 EST by Mrs Leith Woodall on behalf of School of Mathematics & Physics