Genomic organization and regulation of murine alpha haemoglobin stabilizing protein by erythroid Kruppel-like factor

Keys, Janelle R., Tallack, Michael R., Hodge, Denise J., Cridland, Simon O., David, Rakesh and Perkins, Andrew C. (2007) Genomic organization and regulation of murine alpha haemoglobin stabilizing protein by erythroid Kruppel-like factor. British Journal of Haematology, 136 1: 150-156. doi:10.1111/j.1365-2141.2006.06381.x


Author Keys, Janelle R.
Tallack, Michael R.
Hodge, Denise J.
Cridland, Simon O.
David, Rakesh
Perkins, Andrew C.
Title Genomic organization and regulation of murine alpha haemoglobin stabilizing protein by erythroid Kruppel-like factor
Journal name British Journal of Haematology   Check publisher's open access policy
ISSN 0007-1048
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1111/j.1365-2141.2006.06381.x
Volume 136
Issue 1
Start page 150
End page 156
Total pages 7
Place of publication Oxford, England
Publisher Blackwell Publishing
Collection year 2008
Language eng
Subject C1
270207 Quantitative Genetics
730103 Blood disorders
Abstract Alpha haemoglobin stabilising protein (AHSP) binds free alpha-globin chains and plays an important role in the protection of red cells, particularly during beta-thalassaemia. Murine ASHP was discovered as a GATA-1 target gene and human AHSP is directly regulated by GATA-1. More recently, AHSP was rediscovered as a highly erythroid Kruppel-like factor (EKLF) -dependent transcript. We have determined the organisation of the murine AHSP gene and compared it to orthologs. There are two CACC box elements in the proximal promoter. The proximal element is absolutely conserved, but does not bind EKLF as it is not a canonical binding site. In rodents, the distal element contains a 3 bp insertion that disrupts the typical EKLF binding consensus region. Nevertheless, EKLF binds this atypical site by gel mobility shift assay, specifically occupies the AHSP promoter in vivo in a chromatin immunoprecipitation assay, and transactivates AHSP through this CACC site in promoter-reporter assays. Our results suggest EKLF can occupy CACC elements in vivo that are not predictable from the consensus binding site inferred from structural studies. We also propose that absence of AHSP in EKLF-null red cells exacerbates the toxicity of free alpha-globin chains, which exist because of the defect in beta-globin gene activation.
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Tue, 22 Apr 2008, 00:32:23 EST by Cody Mudgway on behalf of Institute for Molecular Bioscience