Sez-6 proteins affect dendritic arborization patterns and excitability of cortical pyramidal neurons

Gunnersen, J. M., Kim, M. H., Fuller, S. J., De Silva, M., Britto, J. M., Hammond, V. E., Davies, P. J., Petrou, S., Faber, E. S. L., Sah, P. and Tan, S. S. (2007) Sez-6 proteins affect dendritic arborization patterns and excitability of cortical pyramidal neurons. Neuron, 56 4: 621-639. doi:10.1016/j.neuron.2007.09.018


Author Gunnersen, J. M.
Kim, M. H.
Fuller, S. J.
De Silva, M.
Britto, J. M.
Hammond, V. E.
Davies, P. J.
Petrou, S.
Faber, E. S. L.
Sah, P.
Tan, S. S.
Title Sez-6 proteins affect dendritic arborization patterns and excitability of cortical pyramidal neurons
Journal name Neuron   Check publisher's open access policy
ISSN 0896-6273
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1016/j.neuron.2007.09.018
Volume 56
Issue 4
Start page 621
End page 639
Total pages 19
Editor K. Brose
E. Marcus
Place of publication United States
Publisher Cell Press
Collection year 2008
Language eng
Subject 320704 Cellular Nervous System
730104 Nervous system and disorders
C1
110903 Central Nervous System
110999 Neurosciences not elsewhere classified
Abstract Development of appropriate dendritic arbors is crucial for neuronal information transfer. We show, using seizure-related gene 6 (sez-6) null mutant mice, that Sez-6 is required for normal dendritic arborization of cortical neurons. Deep-layer pyramidal neurons in the somatosensory cortex of sez-6 null mice exhibit an excess of short dendrites, and cultured cortical neurons lacking Sez-6 display excessive neurite branching. Overexpression of individual Sez-6 isoforms in knockout neurons reveals opposing actions of membrane-bound and secreted Sez-6 proteins, with membrane-bound Sez-6 exerting an antibranching effect under both basal and depolarizing conditions. Layer V pyramidal neurons in knockout brain slices show reduced excitatory postsynaptic responses and a reduced dendritic spine density, reflected by diminished punctate staining for postsynaptic density 95 (PSD-95). In behavioral tests, the sez-6 null mice display specific exploratory, motor, and cognitive deficits. In conclusion, cell-surface protein complexes involving Sez-6 help to sculpt the dendritic arbor, in turn enhancing synaptic connectivity.
Keyword Neurosciences
Gene-expression Patterns
In-vivo
Mental-retardation
Spine Density
Synaptic Plasticity
Hippocampal-neurons
Apical Dendrites
Semaphorin 3a
Adult Cortex
Growth
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Tue, 19 Feb 2008, 01:47:42 EST