A simpler analysis for the measurement of second virial coefficients by self-interaction chromatography

Winzor, D. J., Scott, D. J. and Wills, P. R. (2007) A simpler analysis for the measurement of second virial coefficients by self-interaction chromatography. Analytical Biochemistry, 371 1: 21-25. doi:10.1016/j.ab.2007.08.021


Author Winzor, D. J.
Scott, D. J.
Wills, P. R.
Title A simpler analysis for the measurement of second virial coefficients by self-interaction chromatography
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
Publication date 2007-12-01
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.ab.2007.08.021
Volume 371
Issue 1
Start page 21
End page 25
Total pages 5
Place of publication San Diego
Publisher Academic Press Inc Elsevier Science
Language eng
Abstract We describe a thermodynamic approach that supports the adoption of a simplified procedure for the determination of protein second virial coefficients (B-2) by self-interaction chromatography. Its major advantage over the original method is a decrease in the number of parameters to which magnitudes must be assigned for the determination of B-2. Improved correlation of virial coefficients obtained by the chromatographic procedure with those obtained by light scattering is achieved by taking into account the twofold larger magnitudes of the former because of the experimental distinction between free and immobilized protein molecules in self-interaction chromatography. (c) 2007 Elsevier Inc. All rights reserved.
Keyword Biochemical Research Methods
Biochemistry & Molecular Biology
Chemistry, Analytical
second virial coefficients
self-interaction chromatography
thermodynamic nonideality
lysozyme
Protein-protein Interactions
Affinity-chromatography
Thermodynamic Nonideality
Crystal-growth
Crystallization
Lysozyme
Equilibrium
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Excellence in Research Australia (ERA) - Collection
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 19 Feb 2008, 01:26:34 EST