Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes

Fairbairn, David J., Cavallaro, Antonino S., Bernard, Margaret, Mahalinga-Iyer, Janani, Graham, Michael W. and Botella, José R. (2007) Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes. Planta, 226 6: 1525-1533. doi:10.1007/s00425-007-0588-x


Author Fairbairn, David J.
Cavallaro, Antonino S.
Bernard, Margaret
Mahalinga-Iyer, Janani
Graham, Michael W.
Botella, José R.
Title Host-delivered RNAi: an effective strategy to silence genes in plant parasitic nematodes
Journal name Planta   Check publisher's open access policy
ISSN 0032-0935
1432-2048
Publication date 2007-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1007/s00425-007-0588-x
Open Access Status Not Open Access
Volume 226
Issue 6
Start page 1525
End page 1533
Total pages 9
Place of publication Berlin
Publisher Springer-Verlag
Language eng
Subject C1
270400 Botany
620000 - Plant Production and Plant Primary Products
06 Biological Sciences
Abstract Root-knot nematodes (Meloidogyne spp.) are obligate, sedentary endoparasites that infect many plant species causing large economic losses worldwide. Available nematicides are being banned due to their toxicity or ozone-depleting properties and alternative control strategies are urgently required. We have produced transgenic tobacco (Nicotiana tabacum) plants expressing different dsRNA hairpin structures targeting a root-knot nematode (Meloidogyne javanica) putative transcription factor, MjTis11. We provide evidence that MjTis11 was consistently silenced in nematodes feeding on the roots of transgenic plants. The observed silencing was specific for MjTis11, with other sequence-unrelated genes being unaffected in the nematodes. Those transgenic plants able to induce silencing of MjTis11, also showed the presence of small interfering RNAs. Even though down-regulation of MjTis11 did not result in a lethal phenotype, this study demonstrates the feasibility of silencing root-knot nematode genes by expressing dsRNA in the host plant. Host-delivered RNA interference-triggered (HD-RNAi) silencing of parasite genes provides a novel disease resistance strategy with wide biotechnological applications. The potential of HD-RNAi is not restricted to parasitic nematodes but could be adapted to control other plant-feeding pests.
Formatted abstract
Root-knot nematodes (Meloidogyne spp.) are obligate, sedentary endoparasites that infect many plant species causing large economic losses worldwide. Available nematicides are being banned due to their toxicity or ozone-depleting properties and alternative control strategies are urgently required. We have produced transgenic tobacco (Nicotiana tabacum) plants expressing different dsRNA hairpin structures targeting a root-knot nematode (Meloidogyne javanica) putative transcription factor, MjTis11. We provide evidence that MjTis11 was consistently silenced in nematodes feeding on the roots of transgenic plants. The observed silencing was specific for MjTis11, with other sequence-unrelated genes being unaffected in the nematodes. Those transgenic plants able to induce silencing of MjTis11, also showed the presence of small interfering RNAs. Even though down-regulation of MjTis11 did not result in a lethal phenotype, this study demonstrates the feasibility of silencing root-knot nematode genes by expressing dsRNA in the host plant. Host-delivered RNA interference-triggered (HD-RNAi) silencing of parasite genes provides a novel disease resistance strategy with wide biotechnological applications. The potential of HD-RNAi is not restricted to parasitic nematodes but could be adapted to control other plant-feeding pests.
Keyword Plant Sciences
nematode resistance
RNA interference
root knot nematodes
Double-stranded-rna
Arabidopsis-thaliana
Caenorhabditis-elegans
Meloidogyne-incognita
Interference
Expression
Proteins
Resistance
Ingestion
Dsrnas
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Tue, 19 Feb 2008, 01:21:25 EST