Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells

Larina, O., Bhat, P., Pickett, J. A., Launikonis, B. S., Shah, A., Kruger, W. A., Edwardson, J. M. and Thorn, P. (2007) Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells. Molecular Biology of The Cell, 18 9: 3502-3511. doi:10.1091/mbc.E07-01-0024

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Author Larina, O.
Bhat, P.
Pickett, J. A.
Launikonis, B. S.
Shah, A.
Kruger, W. A.
Edwardson, J. M.
Thorn, P.
Title Dynamic regulation of the large exocytotic fusion pore in pancreatic acinar cells
Journal name Molecular Biology of The Cell   Check publisher's open access policy
ISSN 1059-1524
Publication date 2007-01-01
Sub-type Article (original research)
DOI 10.1091/mbc.E07-01-0024
Open Access Status File (Publisher version)
Volume 18
Issue 9
Start page 3502
End page 3511
Total pages 10
Editor Botstein, D
Place of publication Bethesda
Publisher Amer Soc Cell Biology
Language eng
Subject C1
270602 Animal Physiology - Cell
780105 Biological sciences
Abstract Loss of granule content during exocytosis requires the opening of a fusion pore between the secretory granule and plasma membrane. In a variety of secretory cells, this fusion pore has now been shown to subsequently close. However, it is still unclear how pore closure is physiologically regulated and contentious as to how closure relates to granule content loss. Here, we examine the behavior of the fusion pore during zymogen granule exocytosis in pancreatic acinar cells. By using entry of high-molecular-weight dyes from the extracellular solution into the granule lumen, we show that the fusion pore has a diameter of 29-55 nm. We further show that by 5 min after granule fusion, many granules have a closed fusion pore with evidence indicating that pore closure is a prelude to endocytosis and that in granules with a closed fusion pore the chymotrypsinogen content is low. Finally, we show that latrunculin B treatment promotes pore closure, suggesting F-actin affects pore dynamics. Together, our data do not support the classical view in acinar cells that exocytosis ends with granule collapse. Instead, for many granules the fusion pore closes, probably as a transition to endocytosis, and likely involving an F-actin-dependent mechanism.
Keyword Cell Biology
Insulin-secreting Cells
Kiss-and-run
Chromaffin Cells
Tubular System
Mast-cells
Myosin-ii
Membrane
Granules
Release
Actin
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Tue, 19 Feb 2008, 00:55:44 EST