Autotransporter proteins: novel targets at the bacterial cell surface

Wells, T. J., Tree, J. J., Ulett, G. C. and Schembri, M. A. (2007) Autotransporter proteins: novel targets at the bacterial cell surface. Fems Microbiology Letters, 274 2: 163-172. doi:10.1111/j.1574-6968.2007.00833.x

Author Wells, T. J.
Tree, J. J.
Ulett, G. C.
Schembri, M. A.
Title Autotransporter proteins: novel targets at the bacterial cell surface
Journal name Fems Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
Publication date 2007-09-01
Year available 2007
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1111/j.1574-6968.2007.00833.x
Open Access Status
Volume 274
Issue 2
Start page 163
End page 172
Total pages 10
Place of publication Oxford
Publisher Blackwell Publishing
Language eng
Subject 270301 Bacteriology
780105 Biological sciences
0605 Microbiology
Abstract Autotransporter proteins constitute a family of outer membrane/secreted proteins that possess unique structural properties that facilitate their independent transport across the bacterial membrane system and final routing to the cell surface. Autotransporter proteins have been identified in a wide range of Gram-negative bacteria and are often associated with virulence functions such as adhesion, aggregation, invasion, biofilm formation and toxicity. The importance of autotransporter proteins is exemplified by the fact that they constitute an essential component of some human vaccines. Autotransporter proteins contain three structural motifs: a signal sequence, a passenger domain and a translocator domain. Here, the structural properties of the passenger and translocator domains of three type Va autotransporter proteins are compared and contrasted, namely pertactin from Bordetella pertussis, the adhesion and penetration protein (Hap) from Haemophilus influenzae and Antigen 43 (Ag43) from Escherichia coli. The Ag43 protein is described in detail to examine how its structure relates to functional properties such as cell adhesion, aggregation and biofilm formation. The widespread occurrence of autotransporter-encoding genes, their apparent uniform role in virulence and their ability to interact with host cells suggest that they may represent rational targets for the design of novel vaccines directed against Gram-negative pathogens.
Keyword Microbiology
autotransporter protein
antigen 43
Outer-membrane Protein
Enterotoxigenic Escherichia-coli
Mediates Specific Binding
Catarrhalis Strain O35e
Human Epithelial-cells
Antigen 43
Biofilm Formation
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ
Additional Notes This is an article reviewing multiple works or an entire field of research, which should qualify under HERDC 2008. The research was funded by ARC project DP0557615.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
School of Chemistry and Molecular Biosciences
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 67 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 66 times in Scopus Article | Citations
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Created: Tue, 19 Feb 2008, 00:48:57 EST