Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1

Anderson, D. J. and Botella, J. R. (2007) Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1. Plant Cell Reports, 26 9: 1469-1480. doi:10.1007/s00299-007-0356-1


Author Anderson, D. J.
Botella, J. R.
Title Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein beta-subunit AGB1
Formatted title
Expression analysis and subcellular localization of the Arabidopsis thaliana G-protein ß-subunit AGB1
Journal name Plant Cell Reports   Check publisher's open access policy
ISSN 0721-7714
Publication date 2007-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1007/s00299-007-0356-1
Open Access Status
Volume 26
Issue 9
Start page 1469
End page 1480
Total pages 12
Editor Hahne, G.
Gamborg, O. L.
Place of publication Germany
Publisher Springer
Language eng
Subject C1
270400 Botany
620000 - Plant Production and Plant Primary Products
Abstract Heterotrimeric GTP-binding proteins (G-proteins), consisting of G alpha, G beta, and G gamma subunits, function as molecular switches in many eukaryotic signal transduction pathways. In contrast to many eukaryotes, plants contain very few G-protein subunit isoforms that mediate a diverse array of signalling functions. We investigated the contribution of cell type-specific expression and subcellular localization to this multifunctional signalling capacity for the Arabidopsis thaliana G beta subunit, AGB1. Analysis of AGB1 promoter::beta-glucuronidase (GUS) fusions in germinating seeds, seedlings, and flowering plants revealed that AGB1 is widely expressed throughout development in a complex manner. As well as demonstrating similarities to existing Arabidopsis G-protein subunit expression data, several features of the AGB1 expression pattern align closely with known or proposed G-protein functions. A C-terminal AGB1-green fluorescent protein (GFP) fusion was localized at the plasma membrane and in the nucleus of leaf epidermal cells, trichomes and root cells, supporting previous evidence that plant G-protein functionality relies on subcellular compartmentalization.
Keyword Plant Sciences
heterotrimeric G-protein
Arabidopsis thaliana
signal transduction
guard cell
trichome
root cap
Heterotrimeric G-protein
Green Fluorescent Protein
Plasma-membrane
Salicylic-acid
Cell-division
Gamma-subunit
Living Cells
G-alpha
Gene
Plants
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

 
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Created: Tue, 19 Feb 2008, 00:45:10 EST