The role of F9 fimbriae of uropathogenic Escherichia coli in biofilm formation

Ulett, GC, Mabbett, AN, Fung, KC, Webb, RI and Schembri, MA (2007) The role of F9 fimbriae of uropathogenic Escherichia coli in biofilm formation. Microbiology-sgm, 153 7: 2321-2331. doi:10.1099/mic.0.2006/004648-0


Author Ulett, GC
Mabbett, AN
Fung, KC
Webb, RI
Schembri, MA
Title The role of F9 fimbriae of uropathogenic Escherichia coli in biofilm formation
Journal name Microbiology-sgm   Check publisher's open access policy
ISSN 1350-0872
Publication date 2007-03-02
Year available 2007
Sub-type Article (original research)
DOI 10.1099/mic.0.2006/004648-0
Volume 153
Issue 7
Start page 2321
End page 2331
Total pages 11
Place of publication Reading
Publisher Soc General Microbiology
Language eng
Subject 270301 Bacteriology
C1
730101 Infectious diseases
320401 Medical Bacteriology
Abstract Uropathogenic Escherichia coli (UPEC) is the primary cause of urinary tract infection (UTI) in the developed world. The major factors associated with virulence of UPEC are fimbrial adhesins, which mediate attachment to specific receptors, enhance persistence and trigger innate host responses. UPEC produce a range of fimbrial adhesins, with type 1 and P fimbriae of the chaperone-usher subclass being the best characterized. The prototype UPEC strain CFT073 contains ten gene clusters that contain genes characteristic of this class of fimbriae. However, only five of these gene clusters have been characterized in detail. In this study the F9 fimbrial gene cluster (c1931-c1936) from CFT073 has been characterized. The F9 fimbriae-encoding genes were PCR amplified, cloned and expressed in a K-12 background devoid of type 1 fimbriae. While F9 fimbrial expression was not associated with any haemagglutination or cellular adherence properties, a role in biofilm formation was observed. E. coli K-12 cells expressing F9 fimbriae produced a dense and uniform biofilm in both microtitre plate and continuous-flow biofilm model systems. In wild-type UPEC CIFT073, expression of the F9 major subunit-encoding gene was detected during exponential growth in M9 minimal medium. F9 expression could also be detected following selection and enrichment for pellicle growth in a CIFT073fim foc double mutant. The F9 genes appear to be common in UPEC and other types of pathogenic E coli. However, their precise contribution to disease remains to be determined.
Keyword Microbiology
Urinary-tract-infections
F1c Fimbriae
Type-1 Fimbriae
Phase Variation
Cross-talk
Antigen 43
Fim Genes
Expression
Identification
Strains
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Tue, 19 Feb 2008, 00:26:52 EST