Do type 1 fimbriae promote inflammation in the human urinary tract?

Bergsten, G, Wullt, B, Schembri, MA, Leijonhufvud, I and Svanborg, C (2007) Do type 1 fimbriae promote inflammation in the human urinary tract?. Cellular Microbiology, 9 7: 1766-1781. doi:10.1111/j.1462-5822.2007.00912.x


Author Bergsten, G
Wullt, B
Schembri, MA
Leijonhufvud, I
Svanborg, C
Title Do type 1 fimbriae promote inflammation in the human urinary tract?
Journal name Cellular Microbiology   Check publisher's open access policy
ISSN 1462-5814
Publication date 2007-03-13
Sub-type Article (original research)
DOI 10.1111/j.1462-5822.2007.00912.x
Volume 9
Issue 7
Start page 1766
End page 1781
Total pages 16
Place of publication Oxford
Publisher Blackwell Publishing
Language eng
Subject 320401 Medical Bacteriology
270301 Bacteriology
C1
730101 Infectious diseases
Abstract Type 1 fimbriae have been implicated as virulence factors in animal models of urinary tract infection (UTI), but the function in human disease remains unclear. This study used a human challenge model to examine if type 1 fimbriae trigger inflammation in the urinary tract. The asymptomatic bacteriuria strain Escherichia coli 83972, which fails to express type 1 fimbriae, due to a 4.25 kb fimB-fimD deletion, was reconstituted with a functional fim gene cluster and fimbrial expression was monitored through a gfp reporter. Each patient was inoculated with the fim+ or fim- variants on separate occasions, and the host response to type 1 fimbriae was quantified by intraindividual comparisons of the responses to the fim+ or fim- isogens, using cytokines and neutrophils as end-points. Type 1 fimbriae did not promote inflammation and adherence was poor, as examined on exfoliated cells in urine. This was unexpected, as type 1 fimbriae enhanced the inflammatory response to the same strain in the murine urinary tract and as P fimbrial expression by E. coli 83972 enhances adherence and inflammation in challenged patients. We conclude that type 1 fimbriae do not contribute to the mucosal inflammatory response in the human urinary tract.
Keyword Cell Biology
Microbiology
Uropathogenic Escherichia-coli
Gram-negative Bacteria
Tamm-horsfall Protein
Innate Host Response
Asymptomatic Bacteriuria
Human-erythrocytes
Mucosal Surfaces
Epithelial-cells
Fimh Adhesin
Infections
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Tue, 19 Feb 2008, 03:31:26 EST