EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin

Bryant, D. M., Kerr, M. C., Hammond, L. A., Joseph, S. R., Mostov, K. E., Teasdale, R. D. and Stow, J. L. (2007) EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin. Journal of Cell Science, 120 10: 1818-1828. doi:10.1242/jcs.000653

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Author Bryant, D. M.
Kerr, M. C.
Hammond, L. A.
Joseph, S. R.
Mostov, K. E.
Teasdale, R. D.
Stow, J. L.
Title EGF induces macropinocytosis and SNX1-modulated recycling of E-cadherin
Journal name Journal of Cell Science   Check publisher's open access policy
ISSN 0021-9533
Publication date 2007-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1242/jcs.000653
Open Access Status File (Publisher version)
Volume 120
Issue 10
Start page 1818
End page 1828
Total pages 11
Place of publication Cambridge, England
Publisher Company of Biologists
Language eng
Subject C1
270103 Protein Targeting and Signal Transduction
780106 Political science and public policy
Abstract In epithelia, junction proteins are endocytosed for modulation of cell-cell adhesion and cell polarity. In response to growth factors, the cell-cell adhesion protein E-cadherin is internalized from the cell surface with degradation or recycling as potential fates. However, the cellular machinery involved in cadherin internalization and recycling remains controversial. Here we investigated EGF-induced E-cadherin internalization. EGF stimulation of MCF-7 cells resulted in Rac1-modulated macropinocytosis of the E-cadherin-catenin-complex into endosomal compartments that colocalized with EEA1 and the sorting nexin, SNX1. Depletion of cellular SNX1 levels by siRNA resulted in increased intracellular accumulation and turnover of E-cadherin internalized from the cell surface in response to EGF. Moreover, SNX1 was also required for efficient recycling of internalized E-cadherin and re-establishment of epithelial adhesion. Together, these findings demonstrate a role for SNX1 in retrieval of E-cadherin from a degradative endosomal pathway and in membrane trafficking pathways that regulate E-cadherin recycling.
Keyword Cell Biology
sorting nexin
cell-cell adhesion
Epidermal Growth-factor
Tight Junction Proteins
Adherens Junctions
Sorting Nexins
Nuclear Translocation
Induced Endocytosis
Mediated Adhesion
Tumor Progression
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

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Created: Tue, 19 Feb 2008, 03:16:58 EST