The effects of salivary gland extracts from Boophilus microplus ticks on mitogen-stimulated bovine lymphocytes

Turni, C, Lee, RP and Jackson, LA (2007) The effects of salivary gland extracts from Boophilus microplus ticks on mitogen-stimulated bovine lymphocytes. Veterinary Research Communications, 31 5: 545-552. doi:10.1007/s11259-007-3461-2


Author Turni, C
Lee, RP
Jackson, LA
Title The effects of salivary gland extracts from Boophilus microplus ticks on mitogen-stimulated bovine lymphocytes
Journal name Veterinary Research Communications   Check publisher's open access policy
ISSN 0165-7380
Publication date 2007-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1007/s11259-007-3461-2
Open Access Status
Volume 31
Issue 5
Start page 545
End page 552
Total pages 8
Place of publication Dordrecht
Publisher Springer
Language eng
Subject C1
Abstract The effect of salivary gland extract (SGE) from the tick Boophilus microplus was examined in mitogen-stimulated lymphocytes in vitro. SGE was added to lymphocytes of seven cattle together with the mitogens concanavalin A (ConA), phytohaemagglutinin (PHA) and pokeweed mitogen (PWM). Semi-purified B cells from another seven cattle were stimulated with the mitogen lipopolysaccharide (LPS). PHA and ConA stimulated proliferation of lymphocytes to the same extent, but the inhibition due to SGE of Boophilus microplus on the proliferative response stimulated by PHA (39.0% +/- 9.3%) was less than the inhibition of proliferative response stimulated by ConA (75.4% +/- 6.9%). In contrast, SGE of B. microplus stimulated the proliferation of B cells in the presence of LPS in a dose-dependent manner. Enhanced stimulation of B cells by SGE at > 4 mu g in culture was greater than twice that observed when B cells were stimulated by LPS alone. SGE does not have a direct suppressive effect on bovine B cell proliferation; however, in vivo the effectiveness of B cell responses might be influenced by other immune factors, such as cytokine profiles.
Keyword Veterinary Sciences
Boophilus microplus
immunosuppression
lymphocytes
salivary gland
tick
Flow-cytometric Analysis
Cattle
Responses
Mice
Immunosuppression
Responsiveness
Proliferation
Infestation
Ixodidae
Invitro
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
Queensland Alliance for Agriculture and Food Innovation
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 19 Feb 2008, 02:29:38 EST