Gene expression profiling of cells involved in periodontal regeneration

Ivanovski, S., Lichanska, A.M., D'Aniello, E., Xiao, Y. and Waters, M. J. (2007) Gene expression profiling of cells involved in periodontal regeneration. Tissue Engineering, 13 2: 393-404. doi:10.1089/ten.2006.0082


Author Ivanovski, S.
Lichanska, A.M.
D'Aniello, E.
Xiao, Y.
Waters, M. J.
Title Gene expression profiling of cells involved in periodontal regeneration
Journal name Tissue Engineering   Check publisher's open access policy
ISSN 1076-3279
Publication date 2007-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1089/ten.2006.0082
Open Access Status Not yet assessed
Volume 13
Issue 2
Start page 393
End page 404
Total pages 12
Editor Mikos, A. G.
Johnson, P. C.
Place of publication New Rochelle, United States
Publisher Mary Ann Liebert Inc
Language eng
Subject C1
730112 Oro-dental and disorders
320899 Dentistry not elsewhere classified
Abstract Understanding the molecular mechanisms involved in periodontal regeneration is important for the development of more predictable clinical techniques. This study aimed to identify these mechanisms by comparing the gene expression profiles of cells derived from regenerating defects with patient-matched periodontal ligament cells. Gene profiling was carried out via Affymetrix U133A arrays containing probes for 22,000 genes. Robust differences in gene expression were obtained by identifying genes that consistently changed by a minimum of 2-fold. Analysis of molecular function as designated by gene ontology (GO) identified differentially,regulated mechanisms including protein metabolism, tyrosine kinase activity, and skeletal development. The differentially expressed genes could be broadly divided into the categories of protein biosynthesis and turnover, structural constituents of the cytoskeleton and extracellular matrix, and signal transduction. The differential expression of 4 genes (EGR-1, elastin, osteoprotegerin, and IGFBP3) was confirmed via real-time polymerase chain reaction (PCR). Further, the expression of another 2 differentially expressed transcripts, decorin and biglycan, was immunohistochemically confirmed in a periodontal wound healing model and the protein expression was consistent with the pattern of gene expression. This study gives insight into the molecular processes involved in periodontal regeneration and identifies cell markers that are characteristic of regenerating periodontal tissues.
Keyword Biotechnology & Applied Microbiology
Cell Biology
Extracellular-matrix Proteoglycans
Healing Following Implantation
Signal-transduction
Affected Roots
Bone
Fibroblasts
Tissue
Differentiation
Ligament
Defects
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
School of Dentistry Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 19 Feb 2008, 02:20:28 EST