Incorporated sarcolemmal fish oil fatty acids shorten pig ventricular action potentials

Verkerk, A. O., van Ginneken, A. C. G., Berecki, G., den Ruijter, H. M., Schumacher, C. A., Veldkamp, M. W., Baartscheer, A., Casini, S., Opthof, T., Hovenier, R., Fiolet, J. W. T., Zock, P. L. and Coronel, R. (2006) Incorporated sarcolemmal fish oil fatty acids shorten pig ventricular action potentials. Cardiovascular Research, 70 3: 509-520. doi:10.1016/j.cardiores.2006.02.022

Author Verkerk, A. O.
van Ginneken, A. C. G.
Berecki, G.
den Ruijter, H. M.
Schumacher, C. A.
Veldkamp, M. W.
Baartscheer, A.
Casini, S.
Opthof, T.
Hovenier, R.
Fiolet, J. W. T.
Zock, P. L.
Coronel, R.
Title Incorporated sarcolemmal fish oil fatty acids shorten pig ventricular action potentials
Journal name Cardiovascular Research   Check publisher's open access policy
ISSN 0008-6363
Publication date 2006-06-01
Sub-type Article (original research)
DOI 10.1016/j.cardiores.2006.02.022
Open Access Status Not yet assessed
Volume 70
Issue 3
Start page 509
End page 520
Total pages 12
Place of publication Amsterdam
Publisher Elsevier Science Bv
Language eng
Abstract Background: Omega-3 polyunsaturated fatty acids (W-PUFAs) from fish oil reduce the risk of sudden death presumably by preventing life-threatening arrhythmias. Acutely administered omega 3-PUFAs modulate the activity of several cardiac ion channels, but the chronic effects of a diet enriched with fish oil leading to omega 3-PUFA-incorporation into the sarcolemma on membrane currents are unknown. Methods: Pigs received a diet either rich in W-PUFAs or in omega 9-fatty acids for 8 weeks. Ventricular myocytes (VMs) were isolated and used for patch-clamp studies. Results: omega 3-VMs contained higher amounts of omega 3-PUFAs and had a shorter action potential (AP) with a more negative plateau than control VM. In omega 3 VMs, L-type Ca2+ current (I-Ca,I-L) and Na+-Ca2+ exchange current (I-NCX) were reduced by approximately 20% and 60%, respectively, and inward rectifier K+ current (I-KI) and slow delayed rectifier K+ current (I-Ks) were increased by approximately 50% and 70%, respectively, compared to control. Densities of rapid delayed rectifier K+ current, Ca2+-activated Cl- current, and Na+ current (I-Na) were unchanged, although voltage-dependence of IN,, inactivation was more negative in omega 3 VMs. Conclusions: A fish oil diet increases omega 3-PUFA content in the ventricular sarcolemma, decreases I-Ca,I-L and I-NCX, and increases I-KI and I-Ks, resulting in AP shortening. Incorporation of W-PUFAs in the sarcolemma may have consequences for arrhythmias independent of circulating omega 3-PUFAs. (c) 2006 European Society of Cardiology. Published by Elsevier B.V. All rights reserved.
Keyword Cardiac & Cardiovascular Systems
ion channels
ion exchangers
membrane potential
Ca2+ transients
fatty acids
Dietary Supplementation
Chloride Current
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 70 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 76 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sat, 26 Jan 2008, 02:53:07 EST