Glycoprotein G isoforms from some alpha herpesviruses function as broad-spectrum chemokine binding proteins

Bryant, Neil A., Davis-Poynter, Nick, Vanderplasschen, Alain and Alcami, Antonio (2003) Glycoprotein G isoforms from some alpha herpesviruses function as broad-spectrum chemokine binding proteins. Embo Journal, 22 4: 833-846. doi:10.1093/emboj/cdg092


Author Bryant, Neil A.
Davis-Poynter, Nick
Vanderplasschen, Alain
Alcami, Antonio
Title Glycoprotein G isoforms from some alpha herpesviruses function as broad-spectrum chemokine binding proteins
Journal name Embo Journal   Check publisher's open access policy
ISSN 0261-4189
Publication date 2003-02-17
Sub-type Article (original research)
DOI 10.1093/emboj/cdg092
Volume 22
Issue 4
Start page 833
End page 846
Total pages 14
Place of publication Oxford
Publisher Nature Publishing
Language eng
Subject 060506 Virology
Abstract Mimicry of host chemokines and chemokine receptors to modulate chemokine activity is a strategy encoded by beta- and gammaherpesviruses, but very limited information is available on the anti-chemokine strategies encoded by alphaherpesviruses. The secretion of chemokine binding proteins (vCKBPs) has hitherto been considered a unique strategy encoded by poxviruses and gammaherpesviruses. We describe a family of novel vCKBPs in equine herpesvirus 1, bovine herpesvirus 1 and 5, and related alphaherpesviruses with no sequence similarity to chemokine receptors or other vCKBPs. We show that glycoprotein G (gG) is secreted from infected cells, binds a broad range of chemokines with high affinity and blocks chemokine activity by preventing their interaction with specific receptors. Moreover, gG also blocks chemokine binding to glycosaminoglycans, an interaction required for the correct presentation and function of chemokines in vivo. In contrast to other vCKBPs, gG may also be membrane anchored and, consistently, we show chemokine binding activity at the surface of cells expressing full-length protein. These alphaherpesvirus vCKBPs represent a novel family of proteins that bind chemokines both at the membrane and in solution.
Keyword Biochemistry & Molecular Biology
Cell Biology
chemokine
glycosaminoglycan
herpesvirus
immune evasion
inflammation
Simplex Virus Type-1
Bovine Herpesvirus-1
Equine Herpesvirus-4
Heparin-binding
Immune-system
Dna-sequence
Identification
Gene
Cells
Homolog
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Clinical Medical Virology Centre Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 80 times in Thomson Reuters Web of Science Article | Citations
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Created: Sat, 26 Jan 2008, 01:52:45 EST