Driving Cell Seeding Using Surface Acoustic Wave Fluid Actuation

Li, Haiyan, Friend, James R. and Yeo, Leslie Y. (2007). Driving Cell Seeding Using Surface Acoustic Wave Fluid Actuation. In: Peter Jacobs, Tim McIntyre, Matthew Cleary, David Buttsworth, David Mee, Rose Clements, Richard Morgan and Charles Lemckert, 16th Australasian Fluid Mechanics Conference (AFMC). 16th Australasian Fluid Mechanics Conference (AFMC), Gold Coast, Queensland, Australia, (625-629). 3-7 December, 2007.

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Author Li, Haiyan
Friend, James R.
Yeo, Leslie Y.
Title of paper Driving Cell Seeding Using Surface Acoustic Wave Fluid Actuation
Conference name 16th Australasian Fluid Mechanics Conference (AFMC)
Conference location Gold Coast, Queensland, Australia
Conference dates 3-7 December, 2007
Proceedings title 16th Australasian Fluid Mechanics Conference (AFMC)
Place of Publication Brisbane, Australia
Publisher School of Engineering, The University of Queensland
Publication Year 2007
Year available 2007
Sub-type Fully published paper
ISBN 978-1-864998-94-8
Editor Peter Jacobs
Tim McIntyre
Matthew Cleary
David Buttsworth
David Mee
Rose Clements
Richard Morgan
Charles Lemckert
Start page 625
End page 629
Total pages 5
Collection year 2007
Language eng
Abstract/Summary In this paper, we investigate the ability to drive fluid streaming via a surface acoustic wave (SAW) into a porous bioscaffold structure, and to exploit this effect to deliver fluorescent particles/yeast cells into the scaffold as a potential rapid and efficient method for cell seeding in tissue engineering. The results demonstrate that the seeding process takes approximately 10 seconds, much shorter than that if the cell suspension were to perfuse through the scaffold under the effects of gravity alone (approximately 30 mins). By increasing the input power, both the velocity of the fluid flow and the particle seeding efficiency can be enhanced. At 560 mW, fluid velocities of the order 10 mm/s were achieved; in this case, the particle/yeast seeding efficiency is around 92%. In addition to rapid seeding, the SAW streaming induced perfusion is observed to significantly improve the uniformity of the scaffold cell distribution due to greater penetration into the scaffold. Finally, we verify using a methylene violet staining procedure that 80% of the yeast cells seeded by the SAW method within the scaffold remained viable.
Subjects 320000 Medical and Health Sciences
Keyword fluid streaming
surface acoustic wave (SAW)
cell seeding
tissue engineering
Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Conference Paper
Collection: 16th Australasian Fluid Mechanics Conference
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Created: Wed, 19 Dec 2007, 10:19:27 EST by Thelma Whitbourne on behalf of School of Engineering