Isolation of DNA and RNA from ascidians

Kumar, S., Degnan, B. M., Ross, I. L., Hawkins, C. J. and Lavin, M. F. (1988) Isolation of DNA and RNA from ascidians. Marine Biology, 98 1: 95-100. doi:10.1007/BF00392663

Author Kumar, S.
Degnan, B. M.
Ross, I. L.
Hawkins, C. J.
Lavin, M. F.
Title Isolation of DNA and RNA from ascidians
Journal name Marine Biology   Check publisher's open access policy
ISSN 0025-3162
Publication date 1988-05-01
Sub-type Article (original research)
DOI 10.1007/BF00392663
Open Access Status
Volume 98
Issue 1
Start page 95
End page 100
Total pages 6
Place of publication Berlin
Publisher Springer-Verlag
Language eng
Subject 270504 Invertebrate Biology
270200 Genetics
Abstract A procedure is described for the extraction of DNA and RNA from solitary and colonial ascidians obtained on the east coast of Australia. Modifications to more established procedures have been made to avoid nuclease digestion, binding to polyphenolic compounds and other less well characterized pigments that interfere with the use of nucleic acids for restriction-enzyme analysis and gene-cloning. Tissue is homogenized in liquid nitrogen to produce a powder which is washed three times in 0.25 M ammonium acetate, pH 5.0, to remove a variety of contaminants. This is followed by lysis and immediate extraction in a number of different phenol/CHC1Jisoamyl alcohol mixtures. Additional steps are similar to those described in previous methods. Extraction of RNA by this procedure precludes the necessity for addition ofribonuclease inhibitors.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Degnan Laboratory
School of Biological Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 12 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 11 times in Scopus Article | Citations
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Created: Thu, 06 Dec 2007, 02:58:53 EST by Laura McTaggart