Chymotrypsin mRNA expression in digestive gland amoebocytes: cell specification occurs prior to metamorphosis and gut morphogenesis in the gastropod Haliotis rufescens

Degnan, B. M., Groppe, J. C. and Morse, D. E. (1995) Chymotrypsin mRNA expression in digestive gland amoebocytes: cell specification occurs prior to metamorphosis and gut morphogenesis in the gastropod Haliotis rufescens. Development Genes and Evolution, 205 1-2: 97-101. doi:10.1007/BF00188848


Author Degnan, B. M.
Groppe, J. C.
Morse, D. E.
Title Chymotrypsin mRNA expression in digestive gland amoebocytes: cell specification occurs prior to metamorphosis and gut morphogenesis in the gastropod Haliotis rufescens
Journal name Development Genes and Evolution   Check publisher's open access policy
ISSN 0949-944X
1432-041X
Publication date 1995-09-01
Sub-type Other
DOI 10.1007/BF00188848
Open Access Status DOI
Volume 205
Issue 1-2
Start page 97
End page 101
Total pages 5
Place of publication Berlin
Publisher Springer Verlag
Language eng
Subject 270504 Invertebrate Biology
270201 Gene Expression
Abstract In the non-feeding larva of the marine gastropod, Haliotis rufescens, gut morphogenesis is initiated at metamorphosis. Intestine-specific chymotrypsin gene expression begins in amoebocytes located in the dorsoposterior region of the undifferentiated digestive gland prior to metamorphosis, 5 d post-fertilization. Transcript accumulates steadily in these cells over the next 6 d while the amoebocytes migrate slowly dorsally. Induction of metamorphosis dramatically accelerates the rates of chymotrypsin mRNA accumulation and amoebocyte migration, and is required for homing of the amoebocytes to the hindgut region. Induction of chymotrypsin gene expression occurs only in larvae that had developed competence to recognize an exogenous morphogenetic cue and initiate metamorphosis, with a more pronounced increase in chymotrypsin mRNA accumulation in occurring older larvae. Chymotrypsin mRNA accumulation patterns suggest that hindgut cell specification occurs prior to metamorphosis, but that completion of the morphogenetic program requires signaling events associated with metamorphosis.
Keyword Chymotrypsin
Larva
Metamorphosis Mollusc
In situ hybridization
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Other
Collections: Degnan Laboratory
School of Biological Sciences Publications
 
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Created: Thu, 06 Dec 2007, 01:17:25 EST by Laura McTaggart