Microsatellite genotyping of individual abalone larvae: parentage assignment in aquaculture

Selvamani, Maria John P., Degnan, Sandie M. and Degnan, Bernard M. (2001) Microsatellite genotyping of individual abalone larvae: parentage assignment in aquaculture. Marine Biotechnology, 3 5: 478-485. doi:10.1007/s1012601-0062-X


Author Selvamani, Maria John P.
Degnan, Sandie M.
Degnan, Bernard M.
Title Microsatellite genotyping of individual abalone larvae: parentage assignment in aquaculture
Journal name Marine Biotechnology   Check publisher's open access policy
ISSN 1436-2228
1436-2236
Publication date 2001-09-01
Sub-type Article (original research)
DOI 10.1007/s1012601-0062-X
Volume 3
Issue 5
Start page 478
End page 485
Total pages 8
Editor J. Grant Burgess
Place of publication New York
Publisher Springer-Verlag
Language eng
Subject 270504 Invertebrate Biology
270202 Genome Structure
10 Technology
C1
Abstract In aquaculture, microsatellite DNA markers are used to genotype parental broodstock, to assess fertilization success, and to maintain pedigree information for selective breeding. In this study we genotyped individual Haliotis asinina larvae by analyzing a suit of polymorphic microsatellite loci. At least 10 loci can be analyzed from a single abalone veliger larva. We assayed 5 polymorphic loci to identify the parents of individual larvae produced in 3 separate crosses. In all cases, the parents of an individual veliger could be determined from as few as 3 loci. The microsatellite analysis revealed that, in each of our crosses, a single male fathered most of the veligers, despite efforts to normalize the amount of sperm contributed by competing males. These observations suggest that highly controlled breeding practices may be required to ensure that the genetic diversity of an abalone population produced for aquaculture is maintained at the level of diversity of the original broodstock.
Keyword abalone
aquaculture
fertilization
Haliotis asinina
selective breeding
sperm competition
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Degnan Laboratory
School of Biological Sciences Publications
 
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Created: Wed, 05 Dec 2007, 22:14:13 EST by Laura McTaggart on behalf of School of Biological Sciences