Characterization of osteocrin expression in human bone

Bord, S., Ireland, D. C., Moffatt, P., Thomas, G. P. and Compston, J. E. (2005) Characterization of osteocrin expression in human bone. Journal of Histochemistry and Cytochemistry, 53 10: 1181-1187. doi:10.1369/jhc.4C6561.2005

Author Bord, S.
Ireland, D. C.
Moffatt, P.
Thomas, G. P.
Compston, J. E.
Title Characterization of osteocrin expression in human bone
Journal name Journal of Histochemistry and Cytochemistry   Check publisher's open access policy
ISSN 0022-1554
Publication date 2005-10-01
Sub-type Article (original research)
DOI 10.1369/jhc.4C6561.2005
Open Access Status Not Open Access
Volume 53
Issue 10
Start page 1181
End page 1187
Total pages 7
Place of publication Seattle
Publisher Histochemical Society
Language eng
Abstract Csteocrin (Ostn), a bone-active molecule, has been shown in animals to be highly expressed in cells of the osteoblast lineage. We have characterized this protein in human cultured primary human osteoblasts, in developing human neonatal bone, and in iliac crest bone biopsies from adult women. In vivo, Ostn expression was localized in developing human neonatal rib bone, with intense immunoreactivity in osteoblasts on bone-forming surfaces, in newly incorporated osteocytes, and in some late hypertrophic chondrocytes. In adult bone, Ostn expression was specifically localized to osteoblasts and young osteocytes at bone-forming sites. In vitro, Ostn expression decreased time dependently (p < 0.02) in osteoblasts cultured for 2, 3, and 6 days. Expression was further decreased in cultures containing 200 nM hydrocortisone by 1.5-, 2.3-, and 3.1-fold (p < 0.05) at the same time points. In contrast, alkaline phosphatase expression increased with osteoblast differentiation (p < 0.05). Low-dose estradiol decreased Ostn expression time dependently (p < 0.05), whereas Ostn expression in cultures treated with high-dose estradiol was not significantly changed. These results demonstrate that Ostn is expressed in human skeletal tissue, particularly in osteoblasts in developing bone and at sites of bone remodeling, suggesting a role in bone formation. Thus, Ostn provides a marker of osteoblast lineage cells and appears to correlate with osteoblast activity.
Keyword Cell Biology
human bone formation
Cultured Human Osteoblasts
Dose Estrogen Therapy
Matrix Metalloproteinases
Postmenopausal Women
Q-Index Code C1
Additional Notes Established the expression of Osteocrin in human bone thus further underling it’s potential for human therapeutic development. We also demonstrated a correlation with age and hormonal status in female bone samples. My role was to provide the antibodies, carry out PCR confirmation of expression and assist in writing the paper. (Impact factor = 2.513)

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
UQ Diamantina Institute Publications
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 17 Oct 2007, 23:57:56 EST