Fgf signalling is required for formation of cartilage in the head

Walshe, Jennifer and Mason, Ivor (2003) Fgf signalling is required for formation of cartilage in the head. Developmental Biology, 264 2: 522-536. doi:10.1016/j.ydbio.2003.08.010

Author Walshe, Jennifer
Mason, Ivor
Title Fgf signalling is required for formation of cartilage in the head
Journal name Developmental Biology   Check publisher's open access policy
ISSN 0012-1606
ISBN 0012-1606; 1095-564X
Publication date 2003-12-15
Sub-type Article (original research)
DOI 10.1016/j.ydbio.2003.08.010
Open Access Status
Volume 264
Issue 2
Start page 522
End page 536
Total pages 15
Place of publication United States
Publisher Academic Press
Language eng
Subject 06 Biological Sciences
Abstract Characterisation of human craniofacial syndromes and studies in transgenic mice have demonstrated the requirement for Fgf signalling during morphogenesis of membrane bone of the cranium. Here, we report that Fgf activity is also required for development of the oro-pharyngeal skeleton, which develops first as cartilage with some elements subsequently becoming ossified. We show that inhibition of FGF receptor activity in the zebrafish embryo following neural crest emigration from the neural tube results in complete absence of neurocranial and pharyngeal cartilages. Moreover, this Fgf signal is required during a 6-h period soon after initiation of neural crest migration. The spatial and temporal expression of Fgf3 and Fgf8 in pharyngeal endoderm and ventral forebrain and its correlation with patterns of Fgf signalling activity in migrating neural crest makes them candidate regulators of cartilage development. Inhibition of Fgf3 results in the complete absence of cartilage elements that normally form in the third, fourth, fifth, and sixth pharyngeal arches, while those of the first, second, and seventh arches are largely unaffected. Inhibition of Fgf8 alone has variable, but mild, effects. However, inhibition of both Fgf3 and Fgf8 together causes a complete absence of pharyngeal cartilages and the near-complete loss of the neurocranial cartilage. These data implicate Fgf3 and Fgf8 as key regulators of cartilage formation in the vertebrate head.
Keyword Fgf
Pharyngeal arch
Neural crest
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 63 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 72 times in Scopus Article | Citations
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Created: Mon, 06 Feb 2006, 22:53:38 EST