Purification of monoclonal antibodies from cell culture supernatants by Gradiflow (TM) electrophoresis technology

Mahler, Stephen M., Leong, Sharon, Gilbert, Andrew, Yang, Fan, Gray, Peter, Van Dyk, Derek and Roeth, Philip (2006) Purification of monoclonal antibodies from cell culture supernatants by Gradiflow (TM) electrophoresis technology. Journal of Chemical Technology And Biotechnology, 81 3: 445-453. doi:10.1002/jctb.1426


Author Mahler, Stephen M.
Leong, Sharon
Gilbert, Andrew
Yang, Fan
Gray, Peter
Van Dyk, Derek
Roeth, Philip
Title Purification of monoclonal antibodies from cell culture supernatants by Gradiflow (TM) electrophoresis technology
Journal name Journal of Chemical Technology And Biotechnology   Check publisher's open access policy
ISSN 0268-2575
1097-4660
Publication date 2006-03-01
Sub-type Article (original research)
DOI 10.1002/jctb.1426
Open Access Status Not Open Access
Volume 81
Issue 3
Start page 445
End page 453
Total pages 9
Place of publication Chichester, United Kingdom
Publisher John Wiley & Sons for Society of Chemical Industry
Language eng
Subject 1004 Medical Biotechnology
Abstract Monoclonal antibodies (mAbs) are used extensively for analytical, diagnostic and therapeutic applications. The purification of mAbs from cell culture supernatants typically consists of protein A, G or L affinity chromatography, often in association with other conventional chromatographic techniques such as ion exchange and gel filtration. We report the application of Gradiflow (TM) preparative electrophoresis technology, for the separation of mouse and mouse/human chimeric mAbs from cell culture supernatants in their native state. The one-step purification of murine mAb HuLym3 shows that mAbs can be purified from hybridoma cell culture supernatants to high purity, and is thus an alternative to other purification methods based on conventional and affinity chromatography for the production of mAbs for analytical and diagnostic applications. A mouse/human IgG1 chimeric mAb produced by Chinese hamster ovary cells was also purified from cell culture supernatant, and the purity achieved suggests that Gradiflow (TM) electrophoresis could replace affinity chromatography in the downstream processing of mAbs for therapeutic use. Gradiflow (TM) electrophoresis technology is scaleable and thus is applicable to industrial-scale purification of mAbs. (c) 2005 Society of Chemical Industry.
Keyword Biotechnology & applied microbiology
Chemistry, multidisciplinary
Engineering, chemical
Monoclonal antibodies
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ
Additional Notes Published online 25 November 2005

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemical Engineering Publications
 
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Created: Thu, 20 Sep 2007, 03:53:51 EST