Generating controlled molecular gradients in 3D gels

Rosoff, W. J., McAllister, R., Esrick, M. A., Goodhill, G. J. and Urbach, J. S. (2005) Generating controlled molecular gradients in 3D gels. Biotechnology And Bioengineering, 91 6: 754-759. doi:10.1002/bit.20564


Author Rosoff, W. J.
McAllister, R.
Esrick, M. A.
Goodhill, G. J.
Urbach, J. S.
Title Generating controlled molecular gradients in 3D gels
Journal name Biotechnology And Bioengineering   Check publisher's open access policy
ISSN 0006-3592
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1002/bit.20564
Volume 91
Issue 6
Start page 754
End page 759
Total pages 6
Place of publication Hoboken
Publisher John Wiley & Sons Inc
Language eng
Abstract A new method for producing molecular gradients of arbitrary shape in thin three dimensional gels is described. Patterns are produced on the surface of the gel by printing with a micropump that dispenses small droplets of solution at controlled rates. The molecules in the solution rapidly diffuse into the gel and create a smooth concentration profile that is independent of depth. The pattern is relatively stable for long times, and its evolution can be accurately described by finite element modeling of the diffusion equation. As a demonstration of the method, direct measurements of protein gradients are performed by quantitative fluorescence microscopy. A complementary technique for measuring diffusion coefficients is also presented. This rapid, flexible, contactless approach to gradient generation is ideally suited for cell culture experiments to investigate the role of gradients of diffusible substances in processes such as chemotaxis, morphogenesis, and pattern formation, as well as for high-throughput screening of system responses to a wide range of chemical concentrations. (c) 2005 Wiley Periodicals, Inc.
Keyword Biotechnology & Applied Microbiology
cell motility assay
molecular gradients
collagen gel
diffusible factors
Nerve Growth-factor
Neurite Outgrowth
Chemotaxis
Assay
Chamber
Matrix
Fibrin
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Queensland Brain Institute Publications
 
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Created: Thu, 20 Sep 2007, 04:51:46 EST