Measurement of the molecular weight distribution of debranched starch

Castro, J. V., Ward, R. M., Gilbert, R. G. and Fitzgerald, M. A. (2005) Measurement of the molecular weight distribution of debranched starch. Biomacromolecules, 6 4: 2260-2270. doi:10.1021/bm050041t


Author Castro, J. V.
Ward, R. M.
Gilbert, R. G.
Fitzgerald, M. A.
Title Measurement of the molecular weight distribution of debranched starch
Journal name Biomacromolecules   Check publisher's open access policy
ISSN 1525-7797
Publication date 2005-01-01
Year available 2005
Sub-type Article (original research)
DOI 10.1021/bm050041t
Open Access Status DOI
Volume 6
Issue 4
Start page 2260
End page 2270
Total pages 11
Place of publication Washington
Publisher Amer Chemical Soc
Language eng
Abstract Combining molecular weight distribution (MWD) data for linear chains of debranched starch from capillary electrophoresis and from size exclusion chromatography (SEC) with detection by differential refractive index and by multi-angle laser light scattering has enabled Mark-Houwink parameters to be determined for linear starch chains. For accurate results, it was found to be important to take SEC band broadening into account, and a methodology for implementing this is presented. This deconvolution technique can be used to reveal features such as maxima or shoulders in the MWD which have qualitative as well as quantitative significance. Remarkably, these data show that the empirical Mark-Houwink relation between molecular weight and hydrodynamic volume is, for linear debranched starch, valid for much lower molecular weights than synthetic polymers. This implies that these Mark-Houwink parameters can be used with "universal calibration" to enable SEC to be used with relative ease to provide MWDs for debranched starch for essentially any degree of polymerization.
Keyword Biochemistry & Molecular Biology
Chemistry, Organic
Polymer Science
Size-exclusion Chromatography
Gel Permeation Chromatograms
Assisted Carbohydrate Electrophoresis
Molar-based Distribution
Laser-light Scattering
Physicochemical Properties
Viscoelastic Properties
Texture Determinants
Fine-structures
Rice Cultivars
Q-Index Code C1
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Centre for Nutrition and Food Sciences Publications
 
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Created: Thu, 20 Sep 2007, 04:37:48 EST