Ski-interacting Protein, a Bifunctional Nuclear Receptor Coregulator that interacts with N-CoR/SMRT and p300

Leong, Gary M., Subramaniam, Nanthakumar, Issa, Laura L., Barry, Janelle B., Kino, Tomoshige, Driggers, Paul H., Hayman, Michael J., Eisman, John A. and Gardiner, Edith M. (2004) Ski-interacting Protein, a Bifunctional Nuclear Receptor Coregulator that interacts with N-CoR/SMRT and p300. Biochemical And Biophysical Research Communications, 315 4: 1070-1076. doi:10.1016/j.bbrc.2004.02.004


Author Leong, Gary M.
Subramaniam, Nanthakumar
Issa, Laura L.
Barry, Janelle B.
Kino, Tomoshige
Driggers, Paul H.
Hayman, Michael J.
Eisman, John A.
Gardiner, Edith M.
Title Ski-interacting Protein, a Bifunctional Nuclear Receptor Coregulator that interacts with N-CoR/SMRT and p300
Journal name Biochemical And Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2004-03-19
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2004.02.004
Volume 315
Issue 4
Start page 1070
End page 1076
Total pages 7
Place of publication San Diego
Publisher Academic Press Inc Elsevier Science
Language eng
Subject 06 Biological Sciences
0304 Medicinal and Biomolecular Chemistry
0601 Biochemistry and Cell Biology
1101 Medical Biochemistry and Metabolomics
Abstract Ski-interacting protein (SKIP), a vitamin D receptor (VDR) coactivator, also functions as a repressor in Notch signalling in association with the corepressor SMRT. Here we show that SKIP bifunctionally modulates (activates or represses) Retinoid-X receptor (RXR)- and VDR-dependent gene transcription in a cell line-specific manner, with activation in CV-I and repression in P19 cells. The coactivator function of SKIP in these cells appeared to correlate with the relative level and ratio of expression of N-CoR and p300, with greater SKIP activation in higher p300-expressing and lower N-CoR-expressing cell-lines. C-terminal deletion of SKIP (del334-536aa) was associated with strong activation in both CV-1 and P19 cells. The corepressors N-CoR and SMRT and the coregulator p300 interacted with SKIP through the same N-terminal region (1-200aa). Overall these results suggest that transcriptional action of SKIP may depend on distinct functional domains and cell line-specific interactions with both corepressors and coactivators. (C) 2004 Elsevier Inc. All rights reserved.
Keyword Biochemistry & Molecular Biology
Biophysics
SKIP
NcoA-62
N-CoR/SMRT
p300
nuclear receptor
transcription
vitamin D
retinoid X receptor
Vitamin-d-receptor
Retinoid-x-receptor
Mediated Transcription
Gene-expression
Coactivator
Repression
Family
Cells
Differentiation
Corepressors
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 20 Sep 2007, 04:55:00 EST