Slit2 guides both precrossing and postcrossing callosal axons at the midline in vivo

Shu, T. Z., Sundaresan, V., McCarthy, M. M. and Richards, L. J. (2003) Slit2 guides both precrossing and postcrossing callosal axons at the midline in vivo. Journal of Neuroscience, 23 22: 8176-8184.

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Author Shu, T. Z.
Sundaresan, V.
McCarthy, M. M.
Richards, L. J.
Title Slit2 guides both precrossing and postcrossing callosal axons at the midline in vivo
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 0270-6474
Publication date 2003-01-01
Year available 2003
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 23
Issue 22
Start page 8176
End page 8184
Total pages 9
Place of publication Washington
Publisher Soc Neuroscience
Language eng
Abstract Commissural axons generally cross the midline only once. In the Drosophila nerve cord and mouse spinal cord, commissural axons are guided by Slit only after they cross the midline, where Slit prevents these axons from recrossing the midline. In the developing corpus callosum, Slit2 expressed by the glial wedge guides callosal axons before they cross the midline, as they approach the corticoseptal boundary. These data highlighted a potential difference between the role of Slit2 in guiding commissural axons in the brain compared with the spinal cord. Here, we investigate whether Slit2 also guides callosal axons after they cross the midline. Because such questions cannot be addressed in conventional gene knock-out animals, we used in utero injections of antisense oligonucleotides to specifically deplete Slit2 on only one side of the brain. We used this technique together with a novel in vitro assay of hemisected brain slices to specifically analyze postcrossing callosal axons. We find that in the brain, unlike the spinal cord, Slit2 mediates both precrossing and postcrossing axonal guidance. Depletion of Slit2 on one side of the brain causes axons to defasciculate and, in some cases, to aberrantly enter the septum. Because these axons do not recross the midline, we conclude that the principle function of Slit2 at the cortical midline maybe to channel the axons along the correct path and possibly repel them away from the midline. We find no evidence that Slit2 prevents axons from recrossing the midline in the brain.
Keyword Neurosciences
corpus callosum
axon guidance
cortical development
glial wedge
antisense oligonucleotides
Vertebrate Nervous-system
Mammalian Forebrain
Anterior Commissure
Guidance Receptors
Lateral Position
Robo Receptors
Drosophila Cns
Optic Chiasm
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID NS37792
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 82 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 88 times in Scopus Article | Citations
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Created: Thu, 20 Sep 2007, 04:56:12 EST