Increase in synthesis of human monoclonal antibodies by transfected Sp2/0 myeloma mouse cell line under conditions of microgravity

Foster, L. J. R., Catzel, D., Atwa, S., Zarka, M. and Mahler, S. M. (2003) Increase in synthesis of human monoclonal antibodies by transfected Sp2/0 myeloma mouse cell line under conditions of microgravity. Biotechnology Letters, 25 15: 1271-1274. doi:10.1023/A:1025003800414


Author Foster, L. J. R.
Catzel, D.
Atwa, S.
Zarka, M.
Mahler, S. M.
Title Increase in synthesis of human monoclonal antibodies by transfected Sp2/0 myeloma mouse cell line under conditions of microgravity
Journal name Biotechnology Letters   Check publisher's open access policy
ISSN 0141-5492
1573-6776
Publication date 2003-08-01
Year available 2003
Sub-type Article (original research)
DOI 10.1023/A:1025003800414
Open Access Status
Volume 25
Issue 15
Start page 1271
End page 1274
Total pages 4
Place of publication Netherlands
Publisher Springer
Language eng
Abstract Microgravity can influence cell growth and function. A transfected Sp2/0 myeloma cell line P3A2 producing a human IgG1 anti-TNFalpha monoclonal antibody was cultivated in static culture, spinner flasks and simulated microgravity using a rotating wall vessel bioreactor. Microgravity significantly decreased cell growth ( from 1.7 x 10(6) to 7.9 x 10(5) cells/ml), but facilitated the synthesis of antibodies, (1.8, 1.3 and 0.5 mug of anti-TNFalpha hmAb per 10(6) viable cells for cells cultivated under microgravity, in spinner flasks and static cultures, respectively). The results suggest that microgravity could be applied to improve the specific productivity of cell lines producing potentially important therapeutic proteins.
Keyword Biotechnology & Applied Microbiology
human monoclonal antibodies
microgravity
myeloma
Simulated Microgravity
Culture-system
Tnf-alpha
Growth
Cultivation
Expression
Bioreactor
Cartilage
Antigen
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemical Engineering Publications
 
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Created: Thu, 20 Sep 2007, 03:36:29 EST