The structure of the GGA1-GAT domain reveals the molecular basis for ARF binding and membrane association of GGAs

Collins, Brett M., Watson, Peter J. and Owen, David J. (2003) The structure of the GGA1-GAT domain reveals the molecular basis for ARF binding and membrane association of GGAs. Developmental Cell, 4 3: 321-332. doi:10.1016/S1534-5807(03)00037-6


Author Collins, Brett M.
Watson, Peter J.
Owen, David J.
Title The structure of the GGA1-GAT domain reveals the molecular basis for ARF binding and membrane association of GGAs
Journal name Developmental Cell   Check publisher's open access policy
ISSN 1534-5807
Publication date 2003-03-01
Sub-type Article (original research)
DOI 10.1016/S1534-5807(03)00037-6
Volume 4
Issue 3
Start page 321
End page 332
Total pages 12
Place of publication Oxford
Publisher Elsevier Inc.
Language eng
Subject 0601 Biochemistry and Cell Biology
Abstract The GGAs are a family of clathrin adaptor proteins involved in vesicular transport between the trans-Golgi network and endosomal system. Here we confirm reports that GGAs are targeted to the Golgi via interaction between the GGA-GAT domain and ARF-GTP, and we present the structure of the GAT domain of human GGA1, completing the structural description of the folded domains of GGA proteins. The GGA-GAT domain possesses an all alpha-helical fold with a "paper clip" topology comprising two independent subdomains. Structure-based mutagenesis demonstrates that ARF1-GTP binding by GGAs is exclusively governed by the N-terminal "hook" subdomain, and, using an in vitro recruitment assay, we show that ARF-GTP binding by this small structure is required and sufficient for Golgi targeting of GGAs.
Keyword Cell Biology
Developmental Biology
Adp-ribosylation Factors
Trans-golgi Network
Guanine-nucleotide Exchange
Adaptin Appendage Domain
Ear Homology Domain
Gamma-adaptin
Vhs Domains
3-dimensional Structure
Endosome Transport
Proteins Interact
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 20 Sep 2007, 01:03:02 EST