Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichia coli

Rito-Palomares, M. and Middelberg, A. P. J. (2002) Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichia coli. Journal of Chemical Technology And Biotechnology, 77 9: 1025-1029. doi:10.1002/jctb.673


Author Rito-Palomares, M.
Middelberg, A. P. J.
Title Aqueous two-phase systems for the recovery of a recombinant viral coat protein from Escherichia coli
Journal name Journal of Chemical Technology And Biotechnology   Check publisher's open access policy
ISSN 0268-2575
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1002/jctb.673
Open Access Status Not yet assessed
Volume 77
Issue 9
Start page 1025
End page 1029
Total pages 5
Place of publication W Sussex
Publisher John Wiley & Sons Ltd
Language eng
Subject 290000 Engineering and Technology
Abstract In this study the use of an aqueous two-phase system (ATPS) following the direct chemical extraction of a recombinant viral coat protein, from the cytoplasm of Escherichia coli, is evaluated. The driving force is the need to establish an economically-viable process for the manufacture of a vaccine against human papilloma infection. The partition behaviour of recombinant L1 protein, the major structural protein of the virus, and DNA was investigated in a polyethylene glycol (PEG)phosphate system. An evaluation of system parameters including PEG molecular mass and the concentrations of PEG and phosphate was conducted, to estimate conditions under which the L1 protein and DNA partition to opposite phases. ATPS extraction comprising a volume ratio of 1.00, PEG 1000 (18.0%(w/w)) and phosphate (15.0%(w/w)) provided the conditions for accumulation of DNA into the bottom phase and concentration of L1 protein into the opposite phase (ie partition coefficient of DNA; In K-DNA<0.0 and partition coefficient of L1; In K-L1>2.5). The findings reported here demonstrate the potential of ATPS to recover recombinant protein released from E coli by direct chemical extraction. (C) 2002 Society of Chemical Industry.
Keyword Biotechnology & Applied Microbiology
Chemistry, Multidisciplinary
Engineering, Chemical
aqueous two-phase systems
protein recovery
chemical extraction
Virus-like Particles
Human-papillomavirus
2-phase Systems
Extraction
Parameters
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemical Engineering Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 20 Sep 2007, 01:36:31 EST