Use of adenoviruses to study isoform specificity of G-protein-receptor-coupled Ca2+ signaling in intact epithelial cells

Poronnik, P., Cummins, M. M., O'Mullane, L. M. and Cook, D. I. (2002) Use of adenoviruses to study isoform specificity of G-protein-receptor-coupled Ca2+ signaling in intact epithelial cells. Cell Biochemistry And Biophysics, 36 2-3: 221-233. doi:10.1385/CBB:36:2-3:221


Author Poronnik, P.
Cummins, M. M.
O'Mullane, L. M.
Cook, D. I.
Title Use of adenoviruses to study isoform specificity of G-protein-receptor-coupled Ca2+ signaling in intact epithelial cells
Journal name Cell Biochemistry And Biophysics   Check publisher's open access policy
ISSN 1085-9195
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1385/CBB:36:2-3:221
Open Access Status Not yet assessed
Volume 36
Issue 2-3
Start page 221
End page 233
Total pages 13
Place of publication Totowa
Publisher Humana Press Inc
Language eng
Abstract It is firmly established that the activation of many heptahelical receptors by extracellular agonists leads to the activation of effectors such as phospholipase Cbeta (PLCbeta), the subsequent production of inositol-1,4,5-trisphosphate (IP3), and a resultant increase in intracellular free Ca2+. Heterotrimeric G-Proteins have a critical role in transducing the signal from the heptahelical receptor to PLCbeta and in determining the specificity and duration of the cellular responses. There remain, however, a number of areas of uncertainty regarding the exact mechanisms involved in regulating G-protein-mediated receptor-effector coupling in different cell types. For example, the molecular identity of the G-protein involved and the degree of isoform specificity among G-proteins of the same family and their receptors remains unclear. It is also not known in many cell types whether it is the alpha- or the betagamma-subunits of these G-proteins that activate PLCbeta. In order to address these issues, we have used replication-deficient adenoviruses as a tool to deliver, into intact epithelial cells, transgenes coding for proteins involved in G-protein-coupled receptor signaling pathways.
Keyword Biochemistry & Molecular Biology
Biophysics
Cell Biology
G-protein
epithelial
adenovirus
muscarinic
purinergic-phospholipase C
Replication-deficient Adenoviruses
Heterotrimeric G-proteins
Phospholipase C-beta
Nih 3t3 Cells
Alpha-subunit
Gamma-subunit
Rgs Proteins
<ca2+>(i) Oscillations
Endoplasmic-reticulum
Cardiac-hypertrophy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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Created: Thu, 20 Sep 2007, 01:28:14 EST