Cortical axon guidance by the glial wedge during the development of the corpus callosum

Shu, T. Z. and Richards, L. J. (2001) Cortical axon guidance by the glial wedge during the development of the corpus callosum. Journal of Neuroscience, 21 8: 2749-2758.

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Author Shu, T. Z.
Richards, L. J.
Title Cortical axon guidance by the glial wedge during the development of the corpus callosum
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 0270-6474
Publication date 2001-01-01
Year available 2001
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 21
Issue 8
Start page 2749
End page 2758
Total pages 10
Place of publication Washington
Publisher Soc Neuroscience
Language eng
Subject 270210 Neurogenetics
320702 Central Nervous System
320704 Cellular Nervous System
321005 Fetal Development and Medicine
Abstract Growing axons are often guided to their final destination by intermediate targets. In the developing spinal cord and optic nerve, specialized cells at the embryonic midline act as intermediate targets for guiding commissural axons. Here we investigate whether similar intermediate targets may play a role in guiding cortical axons in the developing brain. During the development of the corpus callosum, cortical axons from one cerebral hemisphere cross the midline to reach their targets in the opposite cortical hemisphere. We have identified two early differentiating populations of midline glial cells that may act as intermediate guideposts for callosal axons. The first differentiates directly below the corpus callosum forming a wedge shaped structure (the glial wedge) and the second differentiates directly above the corpus callosum within the indusium griseum. Axons of the corpus callosum avoid both of these populations in vivo. This finding is recapitulated in vitro in three-dimensional collagen gels. In addition, experimental manipulations in organotypic slices show that callosal axons require the presence and correct orientation of these populations to turn toward the midline. We have also identified one possible candidate for this activity because both glial populations express the chemorepellent molecule slit-2, and cortical axons express the slit-2 receptors robo-1 and robo-2. Furthermore, slit-2 repels-suppresses cortical axon growth in three-dimensional collagen gel cocultures.
Keyword Neurosciences
corpus callosum
axon guidance
glial wedge
cortex development
indusium griseum
Fibrillary Acidic Protein
Neuronal Migration
Cns Midline
Commissure Formation
Anterior Commissure
Embryonic Cns
Optic Chiasm
Fiber Tracts
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID NS37792
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 159 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 20 Sep 2007, 02:22:20 EST