Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining

Nguyen, K. B. and Pender, M. P. (1989) Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining. Stain Technology, 64 4: 163-167. doi:10.3109/10520298909106993

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Author Nguyen, K. B.
Pender, M. P.
Title Assessment of Demyelination in Glycol Methacrylate Sections: a New Protocol for Cresyl Fast Violet Staining
Journal name Stain Technology   Check publisher's open access policy
ISSN 0038-9153
Publication date 1989-01-01
Year available 1989
Sub-type Article (original research)
DOI 10.3109/10520298909106993
Open Access Status File (Author Post-print)
Volume 64
Issue 4
Start page 163
End page 167
Total pages 5
Place of publication Baltimore, MD, United States
Publisher Williams and Wilkins
Language eng
Subject 320602 Cell Physiology
Abstract A simple staining technique for nervous tissue is described. Tissue perfused with glutaraldehyde and formaldehyde and postfixed with osmium tetroxide is embedded in glycol methacrylate. One-micrometer sections are stained with 0.05% cresyl fast violet aqueous solution at 60 C for 5 min, washed with tap water and air dried. With this method the details of all nervous tissue elements are clearly demonstrated. The technique is particularly useful for assessing demyelination because the staining of the axoplasm allows demyelinated axons to be well visualised.
Keyword Demyelination
HistoResin
High resolution light microscopy
Tissue structure
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ
Additional Notes Reprinted from Nguyen, K.B. and M.P. Pender, 'Assessment of demyelination in glycol methacrylate sections: a new protocol for cresyl fast violet staining', Stain Technology, 1989. 64(4): p. 163-7 with permission from Biological Stain Commission (http://www.biostains.org/). The journal 'Stain Technology' is now called 'Biotechnic & Histochemistry' (http://www.louisville.edu/journal/biotech-histochem/).

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Mon, 09 Feb 2004, 10:00:00 EST