The Pineapple EST sequencing and microarray project

Moyle, R. L., Koia, J., Fairbairn, D. J. and Botella, J. R. (2006). The Pineapple EST sequencing and microarray project. In: P.H. Joubert, Acta Horticulturae: Proceedings of the Fifth International Pineapple Symposium. The 5th International Pineapple Symposium, Port Alfred, South Africa, (47-50). 11-16 April 2005. doi:10.17660/ActaHortic.2006.702.5


Author Moyle, R. L.
Koia, J.
Fairbairn, D. J.
Botella, J. R.
Title of paper The Pineapple EST sequencing and microarray project
Conference name The 5th International Pineapple Symposium
Conference location Port Alfred, South Africa
Conference dates 11-16 April 2005
Convener A. Duncan
Proceedings title Acta Horticulturae: Proceedings of the Fifth International Pineapple Symposium   Check publisher's open access policy
Journal name Proceedings of the Vth International Pineapple Symposium   Check publisher's open access policy
Place of Publication Leuven, Belgium
Publisher International Society for Horticultural Science
Publication Year 2006
Year available 2006
Sub-type Fully published paper
DOI 10.17660/ActaHortic.2006.702.5
Open Access Status Not yet assessed
ISBN 978-90-66053-07-6
ISSN 0567-7572
Editor P.H. Joubert
Volume 702
Issue 702
Start page 47
End page 50
Total pages 4
Language eng
Abstract/Summary We have initiated an EST sequencing project to survey a range of expressed sequences from green fruit, yellow fruit, roots, and root-knot nematode infected root/gall tissues. In total, 5681 edited EST sequences were retrieved. Clone redundancy was high in the fruit libraries, with the combined fruit 1548 clone sequences clustering into just 634 contigs comprising 191 consensus sequences and 443 singletons. Half of all fruit EST clone sequences clustered within approximately 14 and 9% of contigs from green unripe and yellow ripe libraries respectively, indicating that a small subset of genes dominates the majority of the transcriptome. The root and root/gall libraries had lower levels of redundancy than the fruit libraries. Half of the root/gall ESTs clustered within approximately 40% of all contigs, indicating the roots possess a more complex transcriptome. Contig assembly and cluster analysis revealed major differences in the abundant gene sequences expressed between the unripe green and the ripe yellow fruit tissues, or gene sequences expressed between the weeks 1-4 and weeks 5-10 nematode infected gall vascular cylinder libraries.
Subjects E1
270201 Gene Expression
620205 Tropical fruit
Keyword fruit ripening
nematode
transgenic pineapple plants
Q-Index Code E1
Institutional Status UQ

 
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Created: Fri, 24 Aug 2007, 08:45:16 EST