Delayed flowering in pineapples (Ananas comosus (L.) Merr.) caused by co-suppression of the ACACS2 gene

Trusov, Y. and Botella, J. R. (2006). Delayed flowering in pineapples (Ananas comosus (L.) Merr.) caused by co-suppression of the ACACS2 gene. In: P.H. Joubert, Acta Horticulturae: Proceedings of the Fifth International Pineapple Symposium. The 5th International Pineapple Symposium, Port Alfred, South Africa, (29-36). 11-16 April 2005. doi:10.17660/ActaHortic.2006.702.2


Author Trusov, Y.
Botella, J. R.
Title of paper Delayed flowering in pineapples (Ananas comosus (L.) Merr.) caused by co-suppression of the ACACS2 gene
Conference name The 5th International Pineapple Symposium
Conference location Port Alfred, South Africa
Conference dates 11-16 April 2005
Convener A. Duncan
Proceedings title Acta Horticulturae: Proceedings of the Fifth International Pineapple Symposium   Check publisher's open access policy
Journal name Proceedings of the Vth International Pineapple Symposium   Check publisher's open access policy
Place of Publication Leuven, Belgium
Publisher International Society for Horticultural Science
Publication Year 2006
Year available 2006
Sub-type Fully published paper
DOI 10.17660/ActaHortic.2006.702.2
Open Access Status Not yet assessed
ISBN 978-90-66053-07-6
ISSN 0567-7572
Editor P.H. Joubert
Volume 702
Issue 702
Start page 29
End page 36
Total pages 8
Language eng
Abstract/Summary Natural flower induction is a major pineapple industry problem. It usually occurs when shortening days and low temperatures give raise to increased ethylene production in the leaf tissue and plant stem apex which in turn stimulates flowering. Natural flowering fruit matures 4 to 6 weeks ahead of the normal summer harvest resulting in the need for extra harvest passes and considerable yield losses. Ethylene is produced through the sequential action of ACC synthase and ACC oxidase. Our team has cloned an ACC synthase gene from pineapple (ACACS2), which is expressed in meristems and activated under the environmental conditions that induce flowering in nature. Genetic constructs have been produced containing ACACS2 in sense orienta¬tion to induce silencing of the host gene in the plant by co-suppression mechanisms. Two independent lines of transgenic plants have been produced and field trials have been conducted in Queensland for four years in order to study the characteristics of the transgenic lines. We have identified a group of transgenic plants demonstrating inherited flowering delay and confirmed co-suppression of the ACACS2 gene due to methylation.
Subjects E1
270402 Plant Physiology
620205 Tropical fruit
Keyword flowering control
transgenic pineapple
ethylene
Q-Index Code E1
Institutional Status UQ

 
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Created: Fri, 24 Aug 2007, 08:45:14 EST