A pore-lining glutamic acid in the olfactory cyclic nucleotide-gated channel controls external spermine block

Nevin, S., Haddrill, J. L. and Lynch, J. W. (2001). A pore-lining glutamic acid in the olfactory cyclic nucleotide-gated channel controls external spermine block. In: , Proceedings of the Australian Neuroscience Society. 21st Annual Meeting of the Australian Neuroscience Society, Brisbane, Australia, (93-93). 28-31 January, 2001.

Author Nevin, S.
Haddrill, J. L.
Lynch, J. W.
Title of paper A pore-lining glutamic acid in the olfactory cyclic nucleotide-gated channel controls external spermine block
Conference Paper Type Fully Published Paper
Conference name 21st Annual Meeting of the Australian Neuroscience Society
Conference location Brisbane, Australia
Conference dates 28-31 January, 2001
Proceedings title Proceedings of the Australian Neuroscience Society
Place published Sydney, N.S.W., Australia
Publisher ANS
Publication date 2001
Volume number 12
ISSN 1034-3237
Start page 93
End page 93
Total pages 1
Collection year 2001
Language eng
Abstract/Summary Spermine is a potent, voltage-dependent blocker of the olfactory cyclic nucleotide-gated channel from both the intracellular and extracellular sides. However, its sites of action are unknown. This study investigated the external spermine binding site in the rat CNC alpha3 subunit. Neutralization of a glutamic acid residue (E342Q) in the P-loop region eliminated voltage-dependence of block by externally applied spermine. The charge-conservative E342D mutation had little effect on spermine block. Thus, E342 forms the binding site for externally applied spermine. However, spermine remained a potent voltage-independent blocker of the E342Q mutant channel, suggesting that the mutation either created a novel binding site outside the membrane electrical field or that it dramatically changed the properties of the existing pore site. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
Subjects EX
320600 Medical Physiology
780105 Biological sciences
Keyword Neurosciences
Polyamine
Spermine
Binding Site
Olfactory
Cyclic Adenosine Monophosphate
Cyclic Nucleotide-gated Channel
Gmp-activated Channel
Cgmp-gated Channel
Divalent-cations
Tiger Salamander
Retinal Rods
Permeation
Polyamines
Rectification
Selectivity
Neurons
Q-Index Code EX

Document type: Conference Paper
Sub-type: Fully Published Paper
Collection: School of Biomedical Sciences Publications
 
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Created: Fri, 24 Aug 2007, 00:50:18 EST