A role for Rho in smooth muscle phenotypic regulation

Campbell, G. R., Worth, N. F. and Rolfe, B. E. (2001). A role for Rho in smooth muscle phenotypic regulation. In: , Annals of the New York Academy of Sciences: Proceedings of the Australian Vascular Biology Society. Australian Vascular Biology Society, Noosa, Queensland, Australia, (316-322). Ausgust, 2001.


Author Campbell, G. R.
Worth, N. F.
Rolfe, B. E.
Title of paper A role for Rho in smooth muscle phenotypic regulation
Conference Paper Type Fully Published Paper
Conference name Australian Vascular Biology Society
Conference location Noosa, Queensland, Australia
Conference dates Ausgust, 2001
Proceedings title Annals of the New York Academy of Sciences: Proceedings of the Australian Vascular Biology Society   Check publisher's open access policy
Place published New York
Publisher The Academy
Publication date 2001
Volume number 947
ISSN 0077-8923
Start page 316
End page 322
Collection year 2001
Language eng
Abstract/Summary The role of the small GTP-binding protein Rho in the process of smooth muscle cell (SMC) phenotypic modulation was investigated using cultured rabbit aortic SMCs. Both Rho transcription and Rho protein expression were high for the first 3 days of culture (contractile state cells), with expression decreasing after change to the synthetic state and peaking upon return to the contractile phenotype. Activation of Rho (indicated by translocation to the membrane) also peaked upon return to the contractile state and was low in synthetic state SMCs. Transient transfection of synthetic state rabbit SMCs with constitutively active Rho (val14rho) caused a dramatic decrease in cell size and reorganization of cytoskeletal proteins to resemble those of the contractile phenotype; alpha-actin and myosin adopted a tightly packed, highly organized arrangement, whereas vimentin localized to the immediate perinuclear region and focal adhesions were enlarged. Conversely, specific inhibition of endogenous Rho, by expression of C3 transferase, resulted in the complete loss of actin and myosin filaments without affecting the distribution of vimentin. Focal adhesions were reduced in number. Thus, Rho plays a key role in regulating SMC phenotypic expression.
Subjects EX
321003 Cardiology (incl. Cardiovascular Diseases)
730106 Cardiovascular system and diseases
Q-Index Code EX
Additional Notes Source: ATHEROSCLEROSIS VI Book Series: ANNALS OF THE NEW YORK ACADEMY OF SCIENCES Volume: 947 Pages: 316-322 Published: 2001

Document type: Conference Paper
Sub-type: Fully Published Paper
Collection: School of Biomedical Sciences Publications
 
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Created: Fri, 24 Aug 2007, 00:39:50 EST