Apoptosis of αβ T lymphocytes in the nervous system in experimental autoimmune encephalomyelitis: Its possible implications for recovery and acquired tolerance

Pender, Michael P., McCombe, Pamela A., Yoong, Gerald and Nguyen, Kim B. (1992) Apoptosis of αβ T lymphocytes in the nervous system in experimental autoimmune encephalomyelitis: Its possible implications for recovery and acquired tolerance. Journal of Autoimmunity, 5 4: 401-410. doi:10.1016/0896-8411(92)90001-7

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
mp_ja_5_92.pdf Author copy application/pdf 412.60KB 406

Author Pender, Michael P.
McCombe, Pamela A.
Yoong, Gerald
Nguyen, Kim B.
Title Apoptosis of αβ T lymphocytes in the nervous system in experimental autoimmune encephalomyelitis: Its possible implications for recovery and acquired tolerance
Journal name Journal of Autoimmunity   Check publisher's open access policy
ISSN 0896-8411
1095-9157
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1016/0896-8411(92)90001-7
Open Access Status File (Author Post-print)
Volume 5
Issue 4
Start page 401
End page 410
Total pages 10
Place of publication London, United Kingdom
Publisher Academic Press
Language eng
Subject 320207 Autoimmunity
320702 Central Nervous System
Abstract We have recently shown that apoptosis, an active process of cellular self-destruction, occurs in the central nervous system in Lewis rats with acute experimental autoimmune encephalomyelitis (EAE) induced by inoculation with myelin basic protein (MBP) and adjuvants. Conventional light and electron microscopic studies suggested that some of the apoptotic cells were oligodendrocytes and that others were hematogenous mononuclear cells. To determine whether any of the apoptotic cells were T lymphocytes, we used the technique of pre-embedding immunolabelling which allows sufficient preservation of the ultrastructure to permit recognition of apoptotic changes while at the same time preserving surface antigens so that the identity of the apoptotic cells can be determined by immunocytochemistry. Light microscopic immunocytochemistry using the mono-clonal antibodies OX-34 (CD2) and R73 (alpha beta T-cell receptor) revealed that 10% of the CD2+ cells and 5% of the alpha beta T lymphocytes in the parenchyma of the spinal cord were dying by apoptosis. The presence of apoptotic alpha beta T cells was confirmed by electron microscopy. About half of all the apoptotic cells within the spinal cord were labelled by these antibodies. It is possible that some of the unlabelled apoptotic cells were also T lymphocytes but that others were glial cells such as oligodendrocytes. One possible interpretation of this T-cell apoptosis is that it represents activation-induced cell death, which has recently been shown to provide a mechanism of clonal elimination of mature as well as immature autoreactive T cells. Another possible interpretation is that it is a result of corticosterone released during the course of EAE. The apoptotic elimination of target-antigen-specific lymphocytes within the target organ in this autoimmune disease may contribute to the subsidence of inflammation and, if ongoing, to the development of tolerance.
Keyword Apoptosis
Experimental autoimmune encephalomyelitis
EAE
T lymphocytes
Lewis rat
Cell death
Experimental allergic encephalomyelitis
Q-Index Code C1
Additional Notes Author copy attached, originally published as Michael P. Pender, Pamela A. McCombe, Gerald Yoong and Kim B. Nguyen (1992) Apoptosis of alpha beta T Lymphocytes in the Nervous System in Experimental Autoimmune Encephalomyelitis: Its Possible Implications for Recovery and Acquired Tolerance, Journal of Autoimmunity (1992) 5 (4) : 401-410. Copyright 1992 Academic Press. All rights reserved.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 107 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 102 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 20 Apr 2005, 10:00:00 EST