Clinical And Histological Findings in Proteolipid Protein-Induced Experimental Autoimmune Encephalomyelitis (EAE) In The Lewis Rat. Distribution of Demyelination Differs From That in EAE Induced by Other Antigens

Chalk, Jonathan B., McCombe, Pamela A., Smith, Ross and Pender, Michael P. (1994) Clinical And Histological Findings in Proteolipid Protein-Induced Experimental Autoimmune Encephalomyelitis (EAE) In The Lewis Rat. Distribution of Demyelination Differs From That in EAE Induced by Other Antigens. Journal of the Neurological Sciences, 123 1-2: 154-161. doi:10.1016/0022-510X(94)90218-6


Author Chalk, Jonathan B.
McCombe, Pamela A.
Smith, Ross
Pender, Michael P.
Title Clinical And Histological Findings in Proteolipid Protein-Induced Experimental Autoimmune Encephalomyelitis (EAE) In The Lewis Rat. Distribution of Demyelination Differs From That in EAE Induced by Other Antigens
Journal name Journal of the Neurological Sciences   Check publisher's open access policy
ISSN 0022-510X
Publication date 1994-05-01
Sub-type Article (original research)
DOI 10.1016/0022-510X(94)90218-6
Volume 123
Issue 1-2
Start page 154
End page 161
Total pages 8
Language eng
Subject 320200 Immunology
321013 Neurology and Neuromuscular Diseases
Abstract Proteolipid protein (PLP) is the major protein of central nervous system (CNS) myelin. In some species, intradermal inoculation with PLP and adjuvants causes experimental autoimmune encephalomyelitis (PLP-EAE) characterized by neurological signs of tail and limb weakness and by inflammation and demyelination in the CNS. A previous study found that inoculation of Lewis rats with 100 microg of PLP causes PLP-EAE with a low incidence of neurological signs and a highly variable clinical course. In the present study we assessed PLP-EAE produced by inoculation with 1000 microg of PLP per rat. Fifty-one of 59 (86%) Lewis rats developed neurological signs 8 to 20 days (mean = 12.0 plus or minus 2.0) after inoculation with 1000 microg of PLP. In such rats, mononuclear cell infiltrates were present in the brain and spinal cord while primary demyelination occurred mainly in the subpial regions of the spinal cord, especially in the dorsal root entry and ventral root exit zones. The histological findings were compared with those in acute EAE induced in the Lewis rat by inoculation with whole CNS tissue or with myelin basic protein: in PLP-EAE, in contrast to these other models, the disease was essentially restricted to the CNS. This form of EAE should be useful in future studies of the consequences of autoimmunity to PLP.
Keyword demyelination
experimental autoimmune encephalomyelitis
Lewis rat
multiple sclerosis
myelin basic protein
proteolipid protein
experimental allergic encephalomyelitis
EAE
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Fri, 10 Jun 2005, 10:00:00 EST