Towards crystallization using optical tweezers

Singer, Wolfgang, Gibson, Ursula J., Nieminen, Timo A., Heckenberg, Norman R. and Rubinsztein-Dunlop, Halina (2006). Towards crystallization using optical tweezers. In: Derek Abbott, Yuri S. Kivshar, Halina H. Rubinsztein-Dunlop and Shanhui Fan, Proceedings of SPIE. Photonics: Design, Technology, and Packaging II, Brisbane, Australia, (60380B-1-60380B-8). 12 December 2005. doi:10.1117/12.651755

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Author Singer, Wolfgang
Gibson, Ursula J.
Nieminen, Timo A.
Heckenberg, Norman R.
Rubinsztein-Dunlop, Halina
Title of paper Towards crystallization using optical tweezers
Conference name Photonics: Design, Technology, and Packaging II
Conference location Brisbane, Australia
Conference dates 12 December 2005
Proceedings title Proceedings of SPIE   Check publisher's open access policy
Journal name Photonics: Design, Technology, and Packaging II   Check publisher's open access policy
Place of Publication Bellingham, Washington, U.S.A.
Publisher SPIE - International Society for Optical Engineering
Publication Year 2006
Sub-type Fully published paper
DOI 10.1117/12.651755
Open Access Status File (Publisher version)
ISBN 0-8194-6069-9
ISSN 0277-786X
Editor Derek Abbott
Yuri S. Kivshar
Halina H. Rubinsztein-Dunlop
Shanhui Fan
Volume 6038
Start page 60380B-1
End page 60380B-8
Total pages 8
Language eng
Abstract/Summary Recently we have shown that protein crystals could be grown while they were three-dimensionally trapped by optical tweezers. This permitted studies of modifications of single crystals while gradually changing the conditions in the growing solution. Furthermore it allowed the crystals to grow far away from container walls favoring high quality crystal growth. Many protein crystals themselves consist of fairly large molecules, with sizes up to tens of nanometers. Here we present experiments studying the effect of optical trapping potentials on large molecules, with the aim to explore ways to further enhance crystal growth. For this purpose we extended our tweezers setup with a specially developed detection system allowing us to monitor changes in the molecule concentration of a solution. Using polyethylene oxide (PEO) molecule solutions we were able to demonstrate that the trapping potential of an optical trap is sufficient to collect large single molecules. Our results show that the optical trap induces an increase in the molecule concentration in the focal region. As expected only molecules above a certain molecular weight could be manipulated, and the concentration in the focal region depended on the power of the trapping laser. The ability to locally increase the concentration of molecules may be useful in assisting nucleation of crystals. ©2006 COPYRIGHT SPIE--The International Society for Optical Engineering. Downloading of the abstract is permitted for personal use only.
Subjects 240400 Optical Physics
250503 Characterisation of Macromolecules
Keyword optical tweezers
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Created: Mon, 09 Jan 2006, 10:00:00 EST by Timo Nieminen on behalf of School of Mathematics & Physics