Comparative Analysis of Ribonuclease P RNA of the Planctomycetes

Butler, Margaret K. and Fuerst, John A. (2004) Comparative Analysis of Ribonuclease P RNA of the Planctomycetes. International Journal of Systematic and Evolutionary Microbiology, 54 4: 1333-1344. doi:10.1099/ijs.0.03013-0

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Author Butler, Margaret K.
Fuerst, John A.
Title Comparative Analysis of Ribonuclease P RNA of the Planctomycetes
Journal name International Journal of Systematic and Evolutionary Microbiology   Check publisher's open access policy
ISSN 1466-5026
Publication date 2004-07-01
Sub-type Article (original research)
DOI 10.1099/ijs.0.03013-0
Open Access Status File (Author Post-print)
Volume 54
Issue 4
Start page 1333
End page 1344
Total pages 12
Place of publication Reading
Publisher Soc General Microbiology
Language eng
Subject 270100 Biochemistry and Cell Biology
Abstract The planctomycetes, order Planctomycetales, are a distinct phylum of domain Bacteria. Genes encoding the RNA portion of ribonuclease P (RNase P) of some planctomycete members were sequenced and compared with existing database planctomycete sequences. rnpB gene sequences encoding RNase P RNA were generated by a conserved primer PCR strategy for Planctomyces brasiliensis, Planctomyces limnophilus, Pirellula marina, Pirellula staleyi strain ATCC 35122, Isosphaera pallida, one other Isosphaera strain, Gemmata obscuriglobus and three other strains of the Gemmata group. These sequences were aligned against reference bacterial sequences and secondary structures of corresponding RNase P RNAs deduced by a comparative approach. P12 helices were found to be highly variable in length, as were helices P16.1 and P19, when present. RNase P RNA secondary structures of Gemmata isolates were found to have unusual features relative to other planctomycetes, including a long P9 helix and an insert in the P13 helix not found in any other member of domain Bacteria. These unique features are consistent with other unusual properties of this genus, distinguishing it from other bacteria. Phylogenetic analyses indicate that relationships between planctomycetes derived from RNase P RNA are consistent with 16S rRNA-based analyses.
Keyword Microbiology
Comparative Sequence-analysis
Anaerobic Ammonium Oxidation
Order Planctomycetales
Gemmata-obscuriglobus
Escherichia-coli
Gene-sequences
Catalytic Core
Bacteria
Pirellula
Ribozyme
Q-Index Code C1
Additional Notes Citation: M. K. Butler and J. A. Fuerst, Comparative Analysis of Ribonuclease P RNA of the Planctomycetes, Int J Syst Evol Microbiol 2004 54: 1333-1344. DOI: 10.1099/ijs.0.03013-0 Copyright 2004 Society for General Microbiology. All rights reserved.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 03 Feb 2006, 10:00:00 EST