Focusing in on structural genomics: The University of Queensland structural biology pipeline

Puri, M., Robin, G., Cowieson, N., Forwood, J. K., Listwan, P., Hu, S. H., Guncar, G., Huber, T., Kellie, S., Hume, D. A., Kobe, B. and Martin, J. L. (2006) Focusing in on structural genomics: The University of Queensland structural biology pipeline. Biomolecular Engineering, 23 6: 281-289. doi:10.1016/j.bioeng.2006.09.002

Author Puri, M.
Robin, G.
Cowieson, N.
Forwood, J. K.
Listwan, P.
Hu, S. H.
Guncar, G.
Huber, T.
Kellie, S.
Hume, D. A.
Kobe, B.
Martin, J. L.
Title Focusing in on structural genomics: The University of Queensland structural biology pipeline
Journal name Biomolecular Engineering   Check publisher's open access policy
ISSN 1389-0344
Publication date 2006
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1016/j.bioeng.2006.09.002
Volume 23
Issue 6
Start page 281
End page 289
Total pages 9
Place of publication Amsterdam
Publisher Elsevier
Collection year 2006
Language eng
Subject CX
270202 Genome Structure
780105 Biological sciences
0699 Other Biological Sciences
Abstract The flood of new genomic sequence information together with technological innovations in protein structure determination have led to worldwide structural genomics (SG) initiatives. The goals of SG initiatives are to accelerate the process of protein structure determination, to fill in protein fold space and to provide information about the function of uncharacterized proteins. In the long-term, these outcomes are likely to impact on medical biotechnology and drug discovery, leading to a better understanding of disease as well as the development of new therapeutics. Here we describe the high throughput pipeline established at the University of Queensland in Australia. In this focused pipeline, the targets for structure determination are proteins that are expressed in mouse macrophage cells and that are inferred to have a role in innate immunity. The aim is to characterize the molecular structure and the biochemical and cellular function of these targets by using a parallel processing pipeline. The pipeline is designed to work with tens to hundreds of target gene products and comprises target selection, cloning, expression, purification, crystallization and structure determination. The structures from this pipeline will provide insights into the function of previously uncharacterized macrophage proteins and could lead to the validation of new drug targets for chronic obstructive pulmonary disease and arthritis. (c) 2006 Elsevier B.V. All rights reserved.
Keyword Biochemical Research Methods
Biotechnology & Applied Microbiology
Genetics & Heredity
high throughput crystallography
protein expression
macrophage proteins
structural genomics
Thermotoga-maritima Proteome
Drug Discovery
Functional Genomics
Q-Index Code CX
Additional Notes This document is a journal review.

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 15 Aug 2007, 10:56:20 EST