Evidence for control of splicing by alternative RNA secondary structures in Dipteran homothorax pre-mRNA

Glazov, A., Pheasant, M., Nahkuri, S. and Mattick, J. S. (2006) Evidence for control of splicing by alternative RNA secondary structures in Dipteran homothorax pre-mRNA. RNA Biology, 3 1: 36-39.


Author Glazov, A.
Pheasant, M.
Nahkuri, S.
Mattick, J. S.
Title Evidence for control of splicing by alternative RNA secondary structures in Dipteran homothorax pre-mRNA
Journal name RNA Biology   Check publisher's open access policy
ISSN 1547-6286
Publication date 2006
Sub-type Article (original research)
Volume 3
Issue 1
Start page 36
End page 39
Total pages 4
Place of publication Austin, USA
Publisher Landes Biosciences
Collection year 2006
Language eng
Subject C1
270201 Gene Expression
780106 Political science and public policy
Formatted abstract
In a recent study that identified highly evolutionary conserved sequences in three genomes of Diptera species we described an ultraconserved element found at an internal exon-intron junction of the Drosophila melanogaster homothorax (hth) gene that appeared to be involved the control of hth pre-mRNA splicing. We also discussed a possible role of RNA secondary structure at this site in the regulation of the hth pre-mRNA splicing. In this report we identify a shorter evolutionary conserved intronic element within the hth gene that is located downstream of the first element and has sequence complementarity to it. We demonstrate that intramolecular interactions between these two elements would give rise to alternative RNA secondary structures, which in turn may result in differential control of the homothorax pre-mRNA splicing. We also provide additional comparative genomic data from several newly available insect genomes supporting our original conclusion that these conserved elements are important in the post-transcriptional regulation of homothorax gene expression in Diptera.
Keyword alternative splicing
intron retention
comparative genomics
Drosophila
Anopheles
Culex
Aedes
Apis
Q-Index Code C1

 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 10:55:13 EST